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. 2010 Aug 27:9:53.
doi: 10.1186/1476-069X-9-53.

A prospective cohort study of biomarkers of prenatal tobacco smoke exposure: the correlation between serum and meconium and their association with infant birth weight

Joe M Braun  1 , Julie L Daniels, Charles Poole, Andrew F Olshan, Richard Hornung, John T Bernert, Yang Xia, Cynthia Bearer, Dana Boyd Barr, Bruce P Lanphear
Affiliations

A prospective cohort study of biomarkers of prenatal tobacco smoke exposure: the correlation between serum and meconium and their association with infant birth weight

Joe M Braun et al. Environ Health. .

Abstract

Background: The evaluation of infant meconium as a cumulative matrix of prenatal toxicant exposure requires comparison to established biomarkers of prenatal exposure.

Methods: We calculated the frequency of detection and concentration of tobacco smoke metabolites measured in meconium (nicotine, cotinine, and trans-3'-hydroxycotinine concentrations) and three serial serum cotinine concentrations taken during the latter two-thirds of pregnancy among 337 mother-infant dyads. We estimated the duration and intensity of prenatal tobacco smoke exposure using serial serum cotinine concentrations and calculated geometric mean meconium tobacco smoke metabolite concentrations according to prenatal exposure. We also compared the estimated associations between these prenatal biomarkers and infant birth weight using linear regression.

Results: We detected nicotine (80%), cotinine (69%), and trans-3'-hydroxycotinine (57%) in most meconium samples. Meconium tobacco smoke metabolite concentrations were positively associated with serum cotinine concentrations and increased with the number of serum cotinine measurements consistent with secondhand or active tobacco smoke exposure. Like serum cotinine, meconium tobacco smoke metabolites were inversely associated with birth weight.

Conclusions: Meconium is a useful biological matrix for measuring prenatal tobacco smoke exposure and could be used in epidemiological studies that enroll women and infants at birth. Meconium holds promise as a biological matrix for measuring the intensity and duration of environmental toxicant exposure and future studies should validate the utility of meconium using other environmental toxicants.

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Figures

Figure 1
Figure 1
Geometric mean meconium tobacco smoke metabolite concentration by duration and intensity of prenatal tobacco smoke exposure. Unexposed indicates all three prenatal serum cotinine measurements were <LOD. 1X, 2X, and 3X indicate the number of serum cotinine measurements consistent with secondhand or active tobacco smoke exposure. Error bars represent 95% CI of the geometric mean.
Figure 2
Figure 2
Adjusted geometric mean meconium nicotine concentration by 16 week and birth serum cotinine category*. *-Adjusted for 26 weeks serum cotinine category (< LOD, LOD - 3 ng/mL, and > 3 ng/mL). Error bars represent 95% CI of the geometric mean.
See this image and copyright information in PMC

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