Determination of the protofilament number in microtubules by templates
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- PMID: 2080278
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Determination of the protofilament number in microtubules by templates
Acta Histochem Suppl.
1990.
Abstract
We have used microtubules (MTs) and double-walled microtubules (dwMTs), both fragmented, as templates for MT formation from phosphocellulose-purified tubulin. In both cases the mean protofilament number of the nucleated MTs corresponds to that of the templates. The results confirm the observations of Scheele et al. (J. Mol. Biol. 154, 485, 1982) that templates determine the protofilament number of nucleated MTs.
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