Antiobesity and hypolipidemic effects of lotus leaf hot water extract with taurine supplementation in rats fed a high fat diet

Abstract

Background: Lotus (Nelumbo nucifera) leaf has been used to treat obesity. The purpose of this study was to investigate the antiobesity and hypolipidemic effects of lotus leaf hot water extract with taurine supplementation in high fat diet-induced obese rats.

Methods: Four week-old male Sprague-Dawley rats were randomly divided into four groups with 8 rats in each group for a period of 6 weeks (normal diet, N group; high fat diet, HF group; high fat diet + lotus leaf hot water extract, HFL group; high fat diet + lotus leaf hot water extract + taurine, HFLT group). Lotus leaf hot water extract was orally administrated to HFL and HFLT groups and the same amount of distilled water was orally administered (400 mg/kg/day) to N and HF groups. Taurine was supplemented by dissolving in feed water (3% w/v).

Results: The body weight gain and relative weights of epididymal and retroperitoneal adipose tissues were significantly lower in N, HFL and HFLT groups compared to HF group. HFL and HFLT groups showed lower concentrations of total cholesterol, triglyceride and low density lipoprotein cholesterol in serum. HFLT group showed higher the ratio of high density lipoprotein cholesterol/total cholesterol compared to HFL group. HFLT group showed better blood lipid profiles compared to HFL group.

Conclusions: Lotus leaf hot water extract with taurine supplementation showed antiobesity and hypolipidemic effects in high fat diet-induced obese rats, which was more effective than lotus leaf hot water extract alone.

Figure 1

Effect of lotus leaf hot water extract with taurine on body weight Average body weights were analyzed by one-way analysis by one way analysis of variance followed by Duncan’s multiple range tests at p<0.05. Superscript stars means that body weight in HF group was significantly higher compared to other groups. N, normal diet group; HF, high fat diet group; HFL, high fat diet + lotus leaf hot water extract group; HFLT, high fat diet + lotus leaf hot water extract + taurine group.

Figure 2

Effect of lotus leaf hot water extract with taurine on epididymal adipocytes Histological photograph: Histological photograph of epididymal adipocytes is fed a normal rats’ diet or a high fat rats’ diet with supplementation of lotus leaf hot water extract alone or lotus leaf hot water extract with taurine. Magnification × 200. There was morphological change in HF group compared to other groups. N, normal diet group; HF, high fat diet group; HFL, high fat diet + lotus leaf hot water extract group; HFLT, high fat diet + lotus leaf hot water extract + taurine group.

Figure 3

Effect of lotus leaf hot water extract with taurine on epididymal adipocytes Sizes of epididymal adipocytes: Effect of lotus leaf hot water extract with taurine supplementation on sizes of epididymal adipocytes in rats fed a normal diet or a high fat diet. Calculation by stochastic 10 cells by Image-Pro Plus 6.0. Data were presented as mean ± SE. Values with different superscripts are significantly different at p<0.05 by Duncan’s multiple range tests. The size in HF group is significantly bigger compared to other groups. N, normal diet group; HF, high fat diet group; HFL, high fat diet + lotus leaf hot water extract group; HFLT, high fat diet + lotus leaf hot water extract + taurine group.