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. 2010 Nov;54(11):4900-2.
doi: 10.1128/AAC.00594-10. Epub 2010 Aug 30.

A mecA-negative strain of methicillin-resistant Staphylococcus aureus with high-level β-lactam resistance contains mutations in three genes

Ritu Banerjee  1 , Michael Gretes, Christopher Harlem, Li Basuino, Henry F Chambers
Affiliations

A mecA-negative strain of methicillin-resistant Staphylococcus aureus with high-level β-lactam resistance contains mutations in three genes

Ritu Banerjee et al. Antimicrob Agents Chemother. 2010 Nov.

Abstract

We previously generated a ceftobiprole-resistant Staphylococcus aureus strain after high inoculum serial passage of a mecA-negative variant of strain COL (R. Banerjee, M. Gretes, L. Basuino, N. Strynadka, and H. F. Chambers, Antimicrob. Agents Chemother. 52:2089-2096, 2008). Genome resequencing of this strain, CRB, revealed that it differs from its parent by five single-nucleotide polymorphisms in three genes, specifically, those encoding PBP4, a low-molecular-weight penicillin-binding protein, GdpP, a predicted signaling protein, and AcrB, a cation multidrug efflux transporter. CRB displayed resistance to a variety of β-lactams but was hypersusceptible to cefoxitin.

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Figures

FIG. 1.
FIG. 1.
PBP4 crystal structure (gray ribbon) in complex with cefotaxime (purple) as recently reported (21), shows that residues F241 and E183 (blue; mutated in ceftobiprole-resistant strain CRB) are adjacent to the active site. Active-site residues S75, S139, and K259 are shown in green. Noncarbon atoms are colored according to the CPK convention.
See this image and copyright information in PMC

References

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