Comment
. 2010 Sep;7(9):667-8; author reply 668.
doi: 10.1038/nmeth0910-667.
Exhaustive benchmarking of the yeast two-hybrid system
- PMID: 20805792
- PMCID: PMC10332476
- DOI: 10.1038/nmeth0910-667
Item in Clipboard
Comment
Exhaustive benchmarking of the yeast two-hybrid system
Nat Methods.
2010 Sep.
No abstract available
Conflict of interest statement
COMPETING FINANCIAL INTERESTS
The authors declare no competing financial interests.
Figures
Comparison of protein interaction methods used by Braun et
al. and
exhaustive Y2H testing. PRS, positive reference set; RRS, random reference set.
X and Y indicate bait and prey proteins, respectively. For each of 92
interactions, the box color indicates the number of assays (out of four Y2H
assays (Braun Y2H) or four other assays (Braun 4 other methods)) that detect the
interaction for the Braun et al. study and the stringency of the assay
that detected the interaction for our study. White color indicates that the
interaction was not detected.
Comment on
-
An experimentally derived confidence score for binary protein-protein interactions.Nat Methods. 2009 Jan;6(1):91-7. doi: 10.1038/nmeth.1281. Epub 2008 Dec 7. Nat Methods. 2009. PMID: 19060903 Free PMC article.
Similar articles
-
A comparison and optimization of yeast two-hybrid systems.Methods. 2012 Dec;58(4):317-24. doi: 10.1016/j.ymeth.2012.12.001. Epub 2012 Dec 8. Methods. 2012. PMID: 23231818 Free PMC article.
-
Systems biology: Playing by the rules.Nature. 2009 Jul 16;460(7253):417. doi: 10.1038/460417a. Nature. 2009. PMID: 19606150 No abstract available.
-
Quantitative real-time PCR as a sensitive protein-protein interaction quantification method and a partial solution for non-accessible autoactivator and false-negative molecule analysis in the yeast two-hybrid system.Methods. 2012 Dec;58(4):376-84. doi: 10.1016/j.ymeth.2012.09.001. Epub 2012 Sep 13. Methods. 2012. PMID: 22982175
-
Fifteen years of the yeast three-hybrid system: RNA-protein interactions under investigation.Methods. 2012 Dec;58(4):367-75. doi: 10.1016/j.ymeth.2012.07.016. Epub 2012 Jul 24. Methods. 2012. PMID: 22841566 Review.
-
Yeast two-hybrid contributions to interactome mapping.Curr Opin Biotechnol. 2006 Aug;17(4):387-93. doi: 10.1016/j.copbio.2006.06.006. Epub 2006 Jun 27. Curr Opin Biotechnol. 2006. PMID: 16806892 Review.
Cited by
-
Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system.Microbiol Mol Biol Rev. 2012 Jun;76(2):331-82. doi: 10.1128/MMBR.05021-11. Microbiol Mol Biol Rev. 2012. PMID: 22688816 Free PMC article. Review.
-
The protein interaction network of bacteriophage lambda with its host, Escherichia coli.J Virol. 2013 Dec;87(23):12745-55. doi: 10.1128/JVI.02495-13. Epub 2013 Sep 18. J Virol. 2013. PMID: 24049175 Free PMC article.
-
The Protein Interactome of Mycobacteriophage Giles Predicts Functions for Unknown Proteins.J Bacteriol. 2015 Aug 1;197(15):2508-16. doi: 10.1128/JB.00164-15. Epub 2015 May 18. J Bacteriol. 2015. PMID: 25986902 Free PMC article.
-
Yeast Two-Hybrid System for Mapping Novel Dengue Protein Interactions.Methods Mol Biol. 2022;2409:119-132. doi: 10.1007/978-1-0716-1879-0_9. Methods Mol Biol. 2022. PMID: 34709639
-
Automated protein-DNA interaction screening of Drosophila regulatory elements.Nat Methods. 2011 Oct 30;8(12):1065-70. doi: 10.1038/nmeth.1763. Nat Methods. 2011. PMID: 22037703 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
