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. 2010 Sep 28;103(7):961-9.
doi: 10.1038/sj.bjc.6605870. Epub 2010 Aug 31.

Ubiquitin D is correlated with colon cancer progression and predicts recurrence for stage II-III disease after curative surgery

D-W Yan  1 , D-W Li, Y-X Yang, J Xia, X-L Wang, C-Z Zhou, J-W Fan, Y-G Wen, H-C Sun, Q Wang, G-Q Qiu, H-M Tang, Z-H Peng
Affiliations

Ubiquitin D is correlated with colon cancer progression and predicts recurrence for stage II-III disease after curative surgery

D-W Yan et al. Br J Cancer. .

Abstract

Background: Our recent study observed that the expression of ubiquitin D (UBD), a member of ubiquitin-like modifier family, was upregulated in colon cancer parenchymal cells. The present study further investigated the clinical signicance of UBD in colon cancer.

Methods: Using quantitative PCR, tissue microarray (TMA), western blot analysis and immunohistochemical stain, we evaluated UBD mRNA and protein levels in tumour tissues from patients with colon cancer at different stages and in paired adjacent normal epithelium.

Results: Immunohistochemical detection of UBD on a TMA containing 203 paired specimens showed that increased cytoplasmic UBD was signicantly associated with depth of cancer invasion, lymph node metastasis, distant metastasis, tumour histologic grade, advanced clinical stage and Ki-67 proliferative index. Patients with UBD-positive tumours had a significantly higher disease recurrence rate and poorer survival than patients with UBD-negative tumours after the radical surgery. Stratification analysis according to tumour stage revealed UBD as an independent predictor for tumour recurrence in patients with stage II and III tumours.

Conclusion: UBD may contribute to the progression of colon carcinogenesis and function as a novel prognostic indicator of forecasting recurrence of stage II and III patients after curative operations.

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Figures

Figure 1
Figure 1
Real-time PCR analysis of UBD mRNA expression in 30 paired colon tumour samples and adjacent normal mucosa. For each sample, the relative UBD mRNA level was normalized using β-actin expression. Data are presented as the median (line) ΔCt value with boxed 25th and 75th percentiles. The data range is represented by the upper and lower bars. ▾P<0.001.
Figure 2
Figure 2
Immunohistochemical staining of Ubiquitin D (UBD) expression in normal tissue and colon cancer. (A, B) Negative UBD expression in normal colonic epithelium (A) and well-differentiated tumour (B). (C) Weak, focal cytoplasmic UBD staining in a well-differentiated colon tumour. (D, E) Diffuse, intense UBD staining in moderately (D) and poorly (E) differentiated colon tumours. (F) Strong UBD staining in a colon cancer lymph node metastasis. Original magnication × 100.
Figure 3
Figure 3
Kaplan–Meier plots of disease-free survival (left) and overall survival (right) of patients with colon cancer who underwent curative resections on the basis of the immunohistochemical UBD expression.
See this image and copyright information in PMC

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