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. 2010 Apr;1(2):106-11.
doi: 10.4103/0974-7788.64401.

A new glycosidic flavonoid from Jwarhar mahakashay (antipyretic) Ayurvedic preparation

Affiliations

A new glycosidic flavonoid from Jwarhar mahakashay (antipyretic) Ayurvedic preparation

Mradu Gupta et al. Int J Ayurveda Res. 2010 Apr.

Abstract

The aqueous extract of Jwarhar mahakashay Ayurvedic preparation (from the roots of Hemidesmus indicus R. Br., Rubia cordifolia L., Cissampelos pareira L.; fruits of Terminalia chebula Retz., Emblica officinalis Gaertn., Terminalia bellirica Roxb., Vitis vinifera L., Grewia asiatica L., Salvadora persica L. and granules of Saccharum officinarum L.) has been used as a traditional antipyretic. Experimental studies confirmed its antipyretic-analgesic effect with very low ulcerogenicity and toxicity. Flavonoids, glycosides and tannins were later found to be present in the extract. Detailed chemical investigations were undertaken after hydrolysis of extract using spectroscopic and chromatography methods to determine its active chemical constituent. UV-Visible spectroscopy showed absorbance maxima at 220 and 276 nm, while fourier transform infra-red investigations indicated an end carboxylic O-H structure at 2940 cm(-1) suggesting the presence of glycoside-linked flavonoids. Thin layer chromatography and high performance liquid chromatography also confirmed the possibility of at least one major and two minor compounds in this abstract. Detailed examination using gas chromatography-mass spectrometry led to the identification of the principal component as 2-(1-oxopropyl)-benzoic acid, which is quite similar to the active compound found in the standard drug Aspirin (2-acetyl-oxybenzoic acid).

Keywords: Antipyretic; Jwarahar mahakashay; chromatography; polyherbal preparation; spectroscopy.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
UV-visible spectroscopic scan of research drug
Figure 2
Figure 2
FTIR finger printing of research drug
Figure 3
Figure 3
HPLC chromatogram of research drug at 220 nm
Figure 4
Figure 4
HPLC chromatogram of research drug at 276 nm
Figure 5
Figure 5
Results of GC–Mass spectroscopy of research drug
Figure 6
Figure 6
Identification of the majority component in research drug using GC–Mass analysis

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