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. 2010 Dec;47(6):1076-9.
doi: 10.1016/j.bone.2010.08.023. Epub 2010 Sep 15.

BoneJ: Free and extensible bone image analysis in ImageJ

Michael Doube  1 , Michał M Kłosowski, Ignacio Arganda-Carreras, Fabrice P Cordelières, Robert P Dougherty, Jonathan S Jackson, Benjamin Schmid, John R Hutchinson, Sandra J Shefelbine
Affiliations

BoneJ: Free and extensible bone image analysis in ImageJ

Michael Doube et al. Bone. 2010 Dec.

Abstract

Bone geometry is commonly measured on computed tomographic (CT) and X-ray microtomographic (μCT) images. We obtained hundreds of CT, μCT and synchrotron μCT images of bones from diverse species that needed to be analysed remote from scanning hardware, but found that available software solutions were expensive, inflexible or methodologically opaque. We implemented standard bone measurements in a novel ImageJ plugin, BoneJ, with which we analysed trabecular bone, whole bones and osteocyte lacunae. BoneJ is open source and free for anyone to download, use, modify and distribute.

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Figures

Figure 1
Figure 1
Graphical output from BoneJ. (A) Trabecular thickness (Tb.Th) measured with the Thickness command. Yellow regions are thicker than blue regions. (B) Centroid and principal axes as calculated by Slice Geometry on a tomographic slice from the tarsometatarsal bone of a little spotted kiwi (Apteryx owenii). (C) Murine osteocyte lacunae imaged with synchrotron μCT and measured with Analyse Particles.
See this image and copyright information in PMC

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