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. 2010 Oct 18;6(20):2246-9.
doi: 10.1002/smll.201000463.

The role of surface functionality on acute cytotoxicity, ROS generation and DNA damage by cationic gold nanoparticles

Apiwat Chompoosor  1 , Krishnendu Saha, Partha S Ghosh, Dylan J Macarthy, Oscar R Miranda, Zheng-Jiang Zhu, Kathleen F Arcaro, Vincent M Rotello
Affiliations

The role of surface functionality on acute cytotoxicity, ROS generation and DNA damage by cationic gold nanoparticles

Apiwat Chompoosor et al. Small. .
No abstract available

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Figures

Figure 1
Figure 1
Cytotoxicity of AuNP 1–4 in HeLa cells determined by alamar blue assay and IC50 of particular AuNPs. The box represents the concentration range used in the ROS generation and DNA damage study.
Figure 2
Figure 2
Quantifications of ROS in HeLa cells determined by the oxidation of H2DCFDA dye. The intracellular gold in each AuNPs was 214 ng/well. The controls were cells alone and cells treated with exogenous H2O2 (0.3% v/v). The data were statistically analyzed and significant ROS level difference was found between AuNP 1 and AuNP 4 (t=12.57, p=0.0002)
Figure 3
Figure 3
Optical images of comet assay a) Cell alone, b) Cell treated with AuNP 1
Figure 4
Figure 4
a) Tail Length of AuNP 1–4 from the comet assay b) % Tail DNA of AuNP 1–4 from the comet assay. Statistical analysis by ANOVA and post hoc t-tests with Bonferoni Correction for multiple comparisons revealed all of the nanoparticles caused significant DNA damage. (p<0.001)
Scheme 1
Scheme 1
A series of AuNPs used in this study and a proposed mechanism of DNA damage determined by comet assay.
See this image and copyright information in PMC

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