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Comparative Study
. 2011 Jan;28(1):15-21.
doi: 10.1007/s10815-010-9475-7. Epub 2010 Sep 4.

Spindle and chromosome configurations of in vitro-matured oocytes from polycystic ovary syndrome and ovulatory infertile women: a pilot study

Rodolpho C Vieira  1 , Ionara D Barcelos, Elisa M Ferreira, Wellington P Martins, Rui A Ferriani, Paula A Navarro
Affiliations
Comparative Study

Spindle and chromosome configurations of in vitro-matured oocytes from polycystic ovary syndrome and ovulatory infertile women: a pilot study

Rodolpho C Vieira et al. J Assist Reprod Genet. 2011 Jan.

Abstract

Purpose: To evaluate the meiotic spindle and chromosomal distribution of in vitro-matured oocytes from infertile nonobese women with PCOS and male or tubal causes of infertility (controls), and to compare in vitro maturation (IVM) rates between groups.

Methods: Seventy four patients (26 with PCOS and 48 controls) undergoing stimulated cycles of oocyte retrieval for ICSI were selected prospectively. Thirteen PCOS patients and 27 controls had immature oocytes retrieved submitted to IVM. After IVM, oocytes showing extrusion of the first polar body were fixed and processed for evaluation of the meiotic spindle and chromosome distribution by immunofluorescence microscopy.

Results: There were no differences between PCOS and control groups with respect to IVM rates (50.0% and 42.9%, respectively) nor the percentage of meiotic abnormalities in metaphase II oocytes (35.3% and 25%, respectively).

Conclusions: In vitro-matured oocytes obtained from stimulated cycles of nonobese PCOS did not have an increased ratio of meiotic abnormalities.

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Figures

Fig. 1
Fig. 1
Fluorescence microscopy images from cell spindle and chromosomes. a Oocyte in normal telophase I showing an elongated cell spindle perpendicular to the oocyte membrane with chromosomes distributed at the ends. b Chromosomes properly aligned in the median portion of a normal barrel-shaped spindle of a metaphase II oocyte. c Chromosome misalignments over a normal barrel-shaped spindle of a metaphase II oocyte. d Chromosome spreading over a normal spindle of a metaphase II oocyte. Green: microtubules forming the cell spindle; Blue: chromosomes. Scale bar = 10 μm
See this image and copyright information in PMC

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