Neural organization in the brainstem circuit mediating the primary acoustic head startle: an electrophysiological study in the rat

Abstract

In awake rats the latency of auditory startle recorded electromyographically in the neck is about 5 ms, suggesting that the primary component of this brainstem reflex is mediated by a neural circuit with only a few synapses. In the present work, neural relays on acoustic head startle circuit are studied in alpha-chloralose-anesthetized rats by means of precise measurements, at putative brainstem relays, of the click-evoked potential latency and of the latency of nuchal EMG startle-like response elicited electrically from each recorded site using the same bipolar electrode. Allowing 0.6 ms for total synaptic transmission time in every relay nuclei, systematic comparisons of the shortest latencies of evoked potentials and shock-elicited startle, as well as estimations of conduction velocities in pathways from cochlea to C1-C5 spinal cord, suggest that one primary acoustic head startle circuit consists of ventral cochlear nucleus (postsynaptic evoked potential: 1.4 ms; startle: 3.6-4.0 ms), ventral nucleus of the lateral lemniscus (evoked potential: 2.3 ms; startle: 2.7-2.8 ms), medial bulbar reticular formation (evoked potential: 3.2-3.6 ms; startle: 2.1 ms), spinal interneuron and motoneuron. The nucleus reticularis pontis caudalis (NRPC) cannot be considered as an head startle relay intercalated between the ventral nucleus of the lateral lemniscus (VNLL) and the medial bulbar reticular formation (MBRF) because mean latencies of field potentials in the pontine RF and the LL nucleus are the same (2.3 ms). Moreover, startle-like responses in neck muscles are elicited through the three brainstem regions with latency differentials which exclude the possibility of a classical synaptic delay either between VNLL (2.8 ms) and NRPC (2.5 ms) or between NRPC and bulbar RF (2.1 ms). Nevertheless, NRPC probably remains a main primary relay on the acoustic startle circuitry; very short latency auditory responses (2.3 ms) are evoked in NRPC by clicks, and low current stimulations of this reticular region produce startle-like activity in neck muscles with a latency of only 2.5 ms. Two other alternative paths consisting of the VCN and NRPC which then would project directly, or through an unknown bulbar site, upon the spinal motor center are hypothetically proposed in conclusion.