[Uracil, contained in DNA, can direct the correction of mispaired nucleotides in human cells]
[Article in
Russian]
- PMID: 2084540
Item in Clipboard
[Uracil, contained in DNA, can direct the correction of mispaired nucleotides in human cells]
[Article in
Russian]
Mol Gen Mikrobiol Virusol.
1990 Dec.
Abstract
The mismatch correction has been studied in human cells and presented in this paper. In the study the experimental model with half-containing hetroduplex (-1 residue in the polylinker region of lac gene) M13 DNA has been used. M 13 DNA was isolated from human cells 24 hours after transfection and transformed into ung+ and ung- cells of Escherichia coli. The percentage of lac- colonies (formed due to frameshift mutation in the lac gene) was analyzed. The increased percent of lac- mutations after human transfection indicated that DNA-uracil can polarize mismatch correction in human cells.
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