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Comparative Study
. 2010 Aug;10(3):204-9.
doi: 10.17305/bjbms.2010.2686.

An immunohistochemical analysis of a rat model of proliferative vitreoretinopathy and a comparison of the expression of TGF-β and PDGF among the induction methods

Xiao-Zhi Zheng  1 , Lian-Fang Du, Hui-Ping Wang
Affiliations
Comparative Study

An immunohistochemical analysis of a rat model of proliferative vitreoretinopathy and a comparison of the expression of TGF-β and PDGF among the induction methods

Xiao-Zhi Zheng et al. Bosn J Basic Med Sci. 2010 Aug.

Abstract

Proliferative vitreoretinopathy (PVR) is a serious complication of retinal detachment surgery or ocular trauma. Our previous study indicated that intravitreal co-injection of retinal pigmented epithelial (RPE)-J cells and platelet-rich plasma (PRP) (not RPE-J cells or PRP alone) in Wistar rat eyes can successfully induce a model of PVR. But which cells are involved in this process and why different induction methods, intravitreal injection of RPE-J cells or/and PRP, induced a different situation remain to be unknown. In this study, immunohistochemistry was performed to identify the main cell types involved in this process. The expression levels of transforming growth factor (TGF)-β2, platelet-derived growth factor (PDGF)-AA and PDGF-BB were tested using enzyme-linked immunosorbent assay (ELISA). The results showed that RPE cells, glial cells, fibroblasts and macrophages took part in the pathogenesis of this model. The expression levels and durations of TGF-β2 and PDGF-BB partially explained the different results induced by the different induction methods. This provides an experimental proof for attenuation of the experimental PVR by targeting at a specific cells or growth factor.

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Figures

FIGURE 1
FIGURE 1
branunohistDchemlcal staining of eyes 14(B, D, F, H) and 28(C, E, G, I) days after co-injection ofPRP and RPE-J ceUs. (A) negative control· (B) and (C) knmunohistochemical staining for cytokeratin-18 showing proUferating RPE ceUs expressing cytokeratin-18; (D) and (E) immunohistochemical staining for gUal fibriUary acidic protein (GFAP) showing gUal ceUs expressing GFAP; (F) and (G) immunohistochemical staining for vimentin showing fibrobksts expressing vimentin; (H) and (I) innnunohistochemical staining for ED1 showing macrophages expressing ED1 (A, B, C, D, E, F,G, H and 1:400x magnification)
FIGURE 2
FIGURE 2
Histogram and curve chart showing the levek ofTGF-ß2(A,B), PDGF-AA(C,D) and PDGF-BB (E,F) expression and their variation with time in the eyes after intravitreal injection ofNS, PKP or/and KPE-J ceDs (*p<0,05) by the detections of growth fectors of every treatment group.
See this image and copyright information in PMC

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