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. 2010 Sep;43(9):604-8.
doi: 10.5483/BMBRep.2010.43.9.604.

Inhibitory effects of antithrombin on the expression of secretory group IIA phospholipase A(2) in endothelial cells

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Free article

Inhibitory effects of antithrombin on the expression of secretory group IIA phospholipase A(2) in endothelial cells

Tae Hoon Kim et al. BMB Rep. 2010 Sep.
Free article

Abstract

Tumor necrosis factor-α (TNF-α) mediates proinflammatory responses in primary human umbilical vein endothelial cells (HUVECs), and it upregulates the expression of secretory group IIA phospholipase A(2) (sPLA(2)-IIA). sPLA(2)-IIA plays a pivotal role in inflammation, and antithrombin (AT) possesses properties that are beneficial to endothelial cells. Therefore, we investigated the effects of AT on the expression of sPLA(2)-IIA in TNF-α-stimulated HUVECs. TNF-α potently upregulated the expression of sPLA(2)-IIA, and prior treatment of cells with AT inhibited the expression of sPLA(2)-IIA in HUVECs. Also, antibodies or siRNA for syndecan-4 blocked the protective effect of AT. Furthermore, PI3-kinase and the AKT pathway are significantly involved in the AT-mediated inhibition of the expression of sPLA(2)-IIA. These results show that AT effectively suppresses the upregulated sPLA(2)-IIA expression, which might contribute to the cytoprotective effects of AT in the treatment of severe inflammatory diseases.

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