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. 2010 Nov;68(3):322-5.
doi: 10.1016/j.diagmicrobio.2010.06.018. Epub 2010 Sep 16.

Detection of metallo-β-lactamase-encoding genes among clinical isolates of Pseudomonas aeruginosa in northwest of Iran

Saber Yousefi  1 , Safar Farajnia, Mohammd Reza Nahaei, Mohammad Taghi Akhi, Reza Ghotaslou, Mohammad Hossein Soroush, Behrooz Naghili, Nima Hosseini Jazani
Affiliations

Detection of metallo-β-lactamase-encoding genes among clinical isolates of Pseudomonas aeruginosa in northwest of Iran

Saber Yousefi et al. Diagn Microbiol Infect Dis. 2010 Nov.

Abstract

The prevalence of metallo-β-lactamase (MBL) production among 104 clinical isolates of Pseudomonas aeruginosa from northwest of Iran was investigated by phenotypic and polymerase chain reaction (PCR) methods. Thirty-nine (37.50%) of isolates were MBL positive by double-disk synergy test. Results of PCR revealed that 18 (17.31%) and 6 (5.77%) imipenem nonsusceptible isolates of P. aeruginosa carried bla(VIM) and bla(IMP) genes respectively, while 92.4% (62/67) of isolates contained class 1 integron gene. This is the first report of MBL-producing P. aeruginosa from northwest of Iran.

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