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. 2010 Nov;12(6):746-9.
doi: 10.2353/jmoldx.2010.100047. Epub 2010 Sep 16.

Genotyping of DNA samples isolated from formalin-fixed paraffin-embedded tissues using preamplification

Renee Baak-Pablo  1 , Vincent Dezentje, Henk-Jan Guchelaar, Tahar van der Straaten
Affiliations

Genotyping of DNA samples isolated from formalin-fixed paraffin-embedded tissues using preamplification

Renee Baak-Pablo et al. J Mol Diagn. 2010 Nov.

Abstract

DNA isolated from formalin-fixed paraffin-embedded (FFPE) tissue is often fragmented and cross-linked and is therefore difficult to genotype. To enable this source of DNA for genotyping analysis using Taqman probes, we tested whether enrichment of the target genes would increase the amount of available DNA. For enrichment of the target genes, we used preamplification by means of diluted Taqman assays. To establish the appropriateness of preamplification, we used DNA extracted from paraffin-embedded tissue and compared the genotyping results of a series of single nucleotide polymorphisms assessed in DNA samples with and without preamplification. In a subset of patients, DNA was isolated from both blood and FFPE tissue to test the reliability of genotyping results derived after preamplification. We found an increase in call rate after preamplification and a convincing concordance in genotype. Based on our findings, we can safely conclude that preamplification of DNA isolated from paraffin-embedded tissue is a valuable and reliable method to optimize genotyping results.

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Figures

Figure 1
Figure 1
Cq values of diluted DNA, directly and preamplified.
Figure 2
Figure 2
Allelic discrimination plots of preamplified DNA isolated from whole blood on the BioMark 48.48 array.
See this image and copyright information in PMC

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