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. 2010 Feb;24(1):14-6.

[Sequence the complete sequence of bocavirus I with SISPA-PCR]

[Article in Chinese]
Jin-Song Li  1 , Wei-Xia Cheng, Dong-Ping Yao, Bei Lan, Jie-Mei Yu, Yan Liu, Yong-Qing Li, Qing Zhang, Miao Jin, Zi-Qian Xu, Dan-Di Li, Zhao-Jun Duan
Affiliations
  • PMID: 20848839

[Sequence the complete sequence of bocavirus I with SISPA-PCR]

[Article in Chinese]
Jin-Song Li et al. Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2010 Feb.

Abstract

Objective: To sequence the complete sequence of bocavirus I with sequence independent single primer amplification (SISPA-PCR).

Methods: To exclude the co-effection samples, all clinical samples of diarrhea cases were screened with special primers of rotavirus, astrovirus, adenovirus, calicivirus and bocavirus I. The virus were enriched through ultracentrifugation. Other nucleic acids, such as human and bacteria genomes, were degradated by DNase I and RNase. DNA of bocavirus was Amplificated with SISPA-PCR, then purificated, cloned and sequenced. The sequences were alighmented in nr with blastn and assembled with DNAstar.

Results: A 4834bp sequence of bocavirus I were assembled.

Conclusion: SISPA-PCR is an economical and efficient technique for sequence a virus complete genome.

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