Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1978;57(2):97-105.
doi: 10.1007/BF01315671.

Inactivation of human cytomegalovirus by phytohemagglutinin

M Ito, L Girvin, A L Barron

Inactivation of human cytomegalovirus by phytohemagglutinin

M Ito et al. Arch Virol. 1978.

Abstract

Human cytomegalovirus (CMV) was inactivated by treatment with phytohemagglutinin (PHA) in contrast to herpes simplex virus (HSV), which was not. Approximately 90% of infectivity was lost following exposure of CMV to PHA. Greater reduction of infectivity, more than 99%, was obtained following pretreatment of cells with PHA than by direct mixture of the virus and the lectin. Protection of cells was still observed 48 hours after pretreatment of cells with PHA. No difference was found in sensitivity to PHA with 3 strains of CMV tested. PHA preparations from different sources were almost equally effective in inactivation of CMV, whereas leucoagglutinin had minimal effect. Our data suggest that the site of action of PHA occurs at the step(s) of penetration and/or uncoating. Resistance of CMV to trypsin was confirmed, and the enzyme had no effect on sensitivity to lectins.

PubMed Disclaimer

References

    1. Nat New Biol. 1971 Sep 8;233(36):50-1 - PubMed
    1. J Virol. 1972 Oct;10(4):844-54 - PubMed
    1. Blood. 1968 Jun;31(6):747-57 - PubMed
    1. Proc Natl Acad Sci U S A. 1973 May;70(5):1308-12 - PubMed
    1. J Immunol. 1972 Mar;108(3):711-8 - PubMed

Publication types