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. 1978 Jun 1;171(3):725-32.
doi: 10.1042/bj1710725.

Substrate, metal and template effects on inhibition of bacteriophage-qbeta ribonucleic acid polymerase by ortho- and pyro-phosphate

R R Brooks, J A Andersen

Substrate, metal and template effects on inhibition of bacteriophage-qbeta ribonucleic acid polymerase by ortho- and pyro-phosphate

R R Brooks et al. Biochem J. .

Abstract

Two reactions of bacteriophage-Qbeta RNA polymerase with synthetic templates were characterized and used to study the effects of substrate, metal and template on inhibition by Pi and PPi. Analysis of the poly(C)-dependent reaction yielded results on kinetics, GTP-dependence, preference for Mn2+ over Mg2+, and Michaelis constants for template similar to those in the literature. New data are provided for the poly(U2,C)-dependent reaction. Our results suggest that GTP and Mn2+ can form relatively stable complexes with the polymerase and that such complexes change the interaction of the enzyme with the inhibitors, Pi and PPi.

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References

    1. J Biol Chem. 1951 Nov;193(1):265-75 - PubMed
    1. Nature. 1970 Nov 7;228(5271):527-33 - PubMed
    1. Jpn J Microbiol. 1971 Mar;15(2):121-9 - PubMed
    1. Nature. 1974 Jun 14;249(458):659-62 - PubMed
    1. Biochem Biophys Res Commun. 1973 Dec 10;55(3):680-8 - PubMed

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