Comparative Study
doi: 10.1016/j.bios.2010.08.038.
Epub 2010 Aug 27.
Evanescent wave fluorescence biosensor combined with DNA bio-barcode assay for platelet genotyping
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- PMID: 20851593
- DOI: 10.1016/j.bios.2010.08.038
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Comparative Study
Evanescent wave fluorescence biosensor combined with DNA bio-barcode assay for platelet genotyping
Biosens Bioelectron.
.
Abstract
An evanescent wave fluorescence biosensor was combined with a DNA bio-barcode assay to resolve problems met in detection of poor biologic samples. Human platelet antigen (HPA) genotyping was used as a demonstrator. Our bio-barcode assay was based on magnetic carboxylatex particles and non-magnetic carboxylatex particles, both functionalized with oligonucleotides. It was assessed for detecting 84mer synthetic oligonucleotides as targets. The assay allows to specifically detect single nucleotide polymorphism with a detection limit of 2 pM of target nucleic acids. The fluorescence detection is achieved in 150 s.
Copyright © 2010 Elsevier B.V. All rights reserved.
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