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Comparative Study
. 2010 Dec 15;26(4):1631-7.
doi: 10.1016/j.bios.2010.08.038. Epub 2010 Aug 27.

Evanescent wave fluorescence biosensor combined with DNA bio-barcode assay for platelet genotyping

Marie Trévisan  1 , Manfred Schawaller, Gerald Quapil, Eliane Souteyrand, Yves Mérieux, Jean-Pierre Cloarec
Affiliations
Comparative Study

Evanescent wave fluorescence biosensor combined with DNA bio-barcode assay for platelet genotyping

Marie Trévisan et al. Biosens Bioelectron. .

Abstract

An evanescent wave fluorescence biosensor was combined with a DNA bio-barcode assay to resolve problems met in detection of poor biologic samples. Human platelet antigen (HPA) genotyping was used as a demonstrator. Our bio-barcode assay was based on magnetic carboxylatex particles and non-magnetic carboxylatex particles, both functionalized with oligonucleotides. It was assessed for detecting 84mer synthetic oligonucleotides as targets. The assay allows to specifically detect single nucleotide polymorphism with a detection limit of 2 pM of target nucleic acids. The fluorescence detection is achieved in 150 s.

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