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. 1990 Dec;191(2):309-13.
doi: 10.1016/0003-2697(90)90224-w.

Use of two-dimensional thin-layer chromatography for the components study of poly(adenosine diphosphate ribose)

G Keith  1 , J Desgrès, G de Murcia
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Use of two-dimensional thin-layer chromatography for the components study of poly(adenosine diphosphate ribose)

G Keith et al. Anal Biochem. 1990 Dec.

Abstract

Two-dimensional thin-layer chromatography on cellulose plates has been used for separating and quantifying the three adenosine derivatives: AMP, phosphoribosyl AMP (PRAMP), and (PR)2AMP obtained by venom phosphodiesterase digestion of poly(ADP-ribose). In vitro synthesized polymer, up to 300 derivatives in length were studied. Some parameters of the complexity of poly(ADP-ribose) could be deduced from our results: (i) The first branching point appears in fragments of approximately 21 derivatives in length. (ii) The branching points are located at regular distances of approximately 41 derivatives from each other.

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