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Comparative Study
. 2010 Nov;76(21):7348-51.
doi: 10.1128/AEM.00942-10. Epub 2010 Sep 17.

Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water

Nicolas Radomski  1 , Françoise S Lucas, Régis Moilleron, Emmanuelle Cambau, Sophie Haenn, Laurent Moulin
Affiliations
Comparative Study

Development of a real-time qPCR method for detection and enumeration of Mycobacterium spp. in surface water

Nicolas Radomski et al. Appl Environ Microbiol. 2010 Nov.

Abstract

A real-time quantitative PCR method was developed for the detection and enumeration of Mycobacterium spp. from environmental samples and was compared to two other methods already described. The results showed that our method, targeting 16S rRNA, was more specific than the two previously published real-time quantitative PCR methods targeting another 16S rRNA locus and the hsp65 gene (100% versus 44% and 91%, respectively).

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