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. 1990 Jun;3(2):118-23.

[Characterization of tumor cell growth inhibitor present in rabbit serum]

[Article in Japanese]
K Miyazaki  1 , T Kimura, T Kakuno
Affiliations
  • PMID: 2085475

[Characterization of tumor cell growth inhibitor present in rabbit serum]

[Article in Japanese]
K Miyazaki et al. Hum Cell. 1990 Jun.

Abstract

Growth inhibitors present in various kinds of sera were surveyed using the rat liver epithelial cell line BRL and its tumorigenic transformant RSV-BRL as indicator cells. This survey revealed that normal rabbit serum contained two types of growth inhibitors: one (GI-A) was more growth-inhibitory on RSV-BRL than BRL, whereas the other (GI-B) vice versa. GI-A was purified 3,000-fold to show a major protein band with Mr 70k on SDS-PAGE. It was an acid-and heat-labile protein and potently inhibited the growth of three kinds of transformed cell lines and two human carcinoma cell lines, but hardly that of non-transformed cell lines, at a dose of 0.5-1.0 micrograms/ml. On the other hand, GI-B was an acid- and heat-stable protein with Mr 25k and was considered to belong to the TGF-beta family.

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