Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Sep 7:5:631-7.
doi: 10.2147/IJN.S12361.

Visualization of gold and platinum nanoparticles interacting with Salmonella enteritidis and Listeria monocytogenes

Ewa Sawosz  1 , André Chwalibog, Jacek Szeliga, Filip Sawosz, Marta Grodzik, Marlena Rupiewicz, Tomasz Niemiec, Katarzyna Kacprzyk
Affiliations

Visualization of gold and platinum nanoparticles interacting with Salmonella enteritidis and Listeria monocytogenes

Ewa Sawosz et al. Int J Nanomedicine. .

Abstract

Purpose: Rapid development of nanotechnology has recently brought significant attention to the extraordinary biological features of nanomaterials. The objective of the present investigation was to evaluate morphological characteristics of the assembles of gold and platinum nanoparticles (nano-Au and nano-Pt respectively), with Salmonella Enteritidis (Gram-negative) and Listeria monocytogenes (Gram-positive), to reveal possibilities of constructing bacteria-nanoparticle vehicles.

Methods: Hydrocolloids of nano-Au or nano-Pt were added to two bacteria suspensions in the following order: nano-Au + Salmonella Enteritidis; nano-Au + Listeria monocytogenes; nano-Pt + Salmonella Enteritidis; nano-Pt + Listeria monocytogenes. Samples were inspected by transmission electron microscope.

Results: Visualization of morphological interaction between nano-Au and Salmonella Enteritidis and Listeria monocytogenes, showed that nano-Au were aggregated within flagella or biofilm network and did not penetrate the bacterial cell. The analysis of morphological effects of interaction of nano-Pt with bacteria revealed that nano-Pt entered cells of Listeria monocytogenes and were removed from the cells. In the case of Salmonella Enteritidis, nano-Pt were seen inside bacteria cells, probably bound to DNA and partly left bacterial cells. After washing and centrifugation, some of the nano-Pt-DNA complexes were observed within Salmonella Enteritidis.

Conclusion: The results indicate that the bacteria could be used as a vehicle to deliver nano-Pt to specific points in the body.

Keywords: bacteria; gold; morphology; nanoparticles; platinum.

PubMed Disclaimer

Figures

Figure 1
Figure 1
TEM image of nanoparticles manufactured by electric non-explosive method: a) gold b) platinum.
Figure 2
Figure 2
TEM image of Salmonella Enteritidis and gold (Au) nanoparticles: a) control; b, c, and d) Salmonella Enteritidis with Au nanoparticles. Arrows point to nano-Au.
Figure 3
Figure 3
TEM image of Listeria monocytogenes and gold (Au) nanoparticles: a) control; b, c, and d) Listeria monocytogenes with Au nanoparticles. Arrows point to nano-Au (b and d); and to aglomerate of nano-Au (c).
Figure 4
Figure 4
TEM image of Salmonella Enteritidis and platinum (Pt) nanoparticles: a) control; b, c, and d) the same cell of Salmonella Enteritidis with Pt nanoparticles. Arrows point to nano-Pt (b); and the spots observed with increasing magnification (b, c, and d). Black spots are seen evaporating under the microscope electron beam and are finally seen as white spots.
Figure 5
Figure 5
TEM image of Listeria monocytogenes and platinum (Pt) nanoparticles: a) control; b, c, and d) Listeria monocytogenes with nano-Pt. Arrows point to nano-PT (b and c); and to black spot partially evaporated under microscope electron beam (b).
Figure 6
Figure 6
TEM image of Salmonella Enteritidis and platinum (Pt) nanoparticles, after washing and centrifugation to remove nanoparticles unattached to bacteria: a) control; b, c, and d) Salmonella Enteritidis with nano-Pt. Arrows point to nano-Pt complexes.
Figure 7
Figure 7
Confocal microscope image of Salmonella Enteritidis: 1) control; 2, 3, and 4) after incubation with platinum nanoparticles for 45, 60, and 90 min respectively. a) Nomarski contrast; b) stained with DAPI; and c) merged pictures.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Ortega J, Vigil CE, Chodkiewicz C. Current progress in targeted therapy for colorectal cancer. Cancer Control. 2010;17(1):7–15. - PubMed
    1. Paukner S, Kohl G, Jalava K, Lubitz W. Sealed bacterial ghosts – novel targeting vehicles for advances drug delivery of water-soluble substances. J Drug Target. 2003;11:151–161. - PubMed
    1. Sawosz E, Grodzik M, Zielińska M, Niemiec T, Olszańska B, Chwalibog A. Nanoparticles of silver do not affect growth, development and DNA oxidative damage in chicken embryos. Arch Geflügelk. 2009;73:208–213.
    1. Sawosz E, Grodzik M, Lisowski P, et al. Hydrocolloids of Ag, Au and Ag/Cu alloy nanoparticles influence inflammatory state at transcriptional level. Bull Vet Inst Pulawy. 2010;54:81–85.
    1. Mukherjee P, Bhattacharya R, Wang P, et al. Antiangiogenic properties of gold nanoparticles. Clin Cancer Res. 2005;11(9):3530–3534. - PubMed

Publication types

MeSH terms

LinkOut - more resources