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. 2010 Oct 25;49(44):8195-200.
doi: 10.1002/anie.201000892.

Noninvasive imaging of dendrimer-type N-glycan clusters: in vivo dynamics dependence on oligosaccharide structure

Katsunori Tanaka  1 , Eric R O Siwu, Kaori Minami, Koki Hasegawa, Satoshi Nozaki, Yousuke Kanayama, Koichi Koyama, Weihsu C Chen, James C Paulson, Yasuyoshi Watanabe, Koichi Fukase
Affiliations

Noninvasive imaging of dendrimer-type N-glycan clusters: in vivo dynamics dependence on oligosaccharide structure

Katsunori Tanaka et al. Angew Chem Int Ed Engl. .
No abstract available

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Figures

Figure 1
Figure 1
Dynamic PET imaging of glycoclusters 1a, 2a, and 3a–c (a–e, respectively) in normal BALB/c nude mice. 68Ga-DOTA-labeled glycoclusters (10 MBq) were administered into the tail vein of the mice (n=3, 500 pmol, 100 μL/mouse) and the whole body was scanned by a small-animal PET scanner, microPET Focus 220 (Siemens Medical Solutions Inc., Knoxville, TN, USA), over 0–4 h after injection; H: heart; K: kidney; L: liver; B: urinary bladder; GB: gallbladder.
Figure 2
Figure 2
a–e) Dynamic fluorescence imaging of glycoclusters 3a–e in BALB/c nude mice: a) 3b; b) 3c; c) 3a; d) 3d; e) 3e. Cy5-labeled glycoclusters 3a–e were administered into the tail vein of the mice (n=3, 500 pmol, 100 μL/mouse) and whole-body scans were performed from the front side by eXplore Optix, GE Healthcare (excitation 646 nm, emission 663 nm) 4 h after injection. Data were normalized. H: heart; L: liver; B: urinary bladder; SP: spleen.
Scheme 1
Scheme 1
Generation and structures of glycoclusters. NBD=nitroben-zoxadiazole.
Scheme 2
Scheme 2
Preparation of N-glycan clusters through histidine-accelerated CuI-mediated Huisgen 1,3-dipolar cycloaddition and labeling by 6π-azaelectrocyclization. Bn=benzyl.
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