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. 2010 Sep 28;16(36):4558-63.
doi: 10.3748/wjg.v16.i36.4558.

High prevalence of viable Mycobacterium avium subspecies paratuberculosis in Crohn's disease

Juan L Mendoza  1 , Amparo San-Pedro, Esther Culebras, Raquel Cíes, Carlos Taxonera, Raquel Lana, Elena Urcelay, Fernando de la Torre, Juan J Picazo, Manuel Díaz-Rubio
Affiliations

High prevalence of viable Mycobacterium avium subspecies paratuberculosis in Crohn's disease

Juan L Mendoza et al. World J Gastroenterol. .

Abstract

Aim: To examine the detection rate of viable Mycobacterium avium subspecies paratuberculosis (MAP) in patients with inflammatory bowel disease [Crohn's disease (CD) and ulcerative colitis (UC)].

Methods: Thirty patients with CD (15 with at least one NOD2/CARD15 mutation), 29 with UC, and 10 with no inflammatory bowel disease (IBD). were tested for MAP by polymerase chain reaction (specific IS900 fragment) and blood culture.

Results: MAP DNA was detected in all original blood samples and 8-wk blood cultures (CD, UC and non-IBD). Positive MAP DNA status was confirmed by dot blot assays. All 69 cultures were negative by acid-fast Ziehl-Neelsen staining. Viable MAP, in spheroplast form, was isolated from the 18-mo blood cultures of all 30 CD patients, one UC patient, and none of the non-IBD controls. No association was found between positive MAP cultures and use of immunosuppressive drugs or CD-associated single nucleotide polymorphisms.

Conclusion: MAP is widely present in our area and MAP DNA can be recovered from the blood of CD, UC and non-IBD patients. However, MAP spheroplasts were only found in CD patients.

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Figures

Figure 1
Figure 1
Microscopic examination of Mycobacterium avium subspecies paratuberculosis cultures isolated from blood of patients with Crohn’s disease. Phenolic-acridine orange techniques to detect cell-wall-deficient Mycobacterium avium subspecies paratuberculosis [spheroplasts (arrow)].
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