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. 2010 Nov 16;22(43):4851-6.
doi: 10.1002/adma.201001525.

Nano-layered microneedles for transcutaneous delivery of polymer nanoparticles and plasmid DNA

Peter C DeMuth  1 , Xingfang Su, Raymond E Samuel, Paula T Hammond, Darrell J Irvine
Affiliations

Nano-layered microneedles for transcutaneous delivery of polymer nanoparticles and plasmid DNA

Peter C DeMuth et al. Adv Mater. .
No abstract available

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Figures

Figure 1
Figure 1
(A) SEM micrograph of uncoated PLGA microneedle arrays of pyramidal geometry (scale - 500μm). (B) Film growth (left axis) and absorbance (right axis) for (Poly-1/pLUC)n multilayers assembled on silicon/quartz substrates bearing a (PS/SPS)20 initiating layer (black bar - (PS/SPS)20, grey bar - (Poly-1/pLUC)n, dashed line – Ab-260 nm). (C, D) Representative confocal micrographs showing a (C) (PS/SPS)20-(Poly-1/Cy3-pLUC)24 coated microneedle and a (D) (PS/SPS)20-(Poly-1/DiI-PLGA NP)4 coated microneedle (left – transverse section, right – lateral sections, 200μm intervals, scale - 200 μm). (E) SEM micrograph showing a (PS/SPS)20-(Poly-1/PLGA NP)4 coated microneedle array (scale - 50μm). (F) Representative confocal micrographs showing a (PS/SPS)20-(Poly-1/Cy3-pLUC)24-(Poly-1/DiD-PLGA NP)4 co-coated microneedle (transverse and lateral sections, left – Cy3-pLUC, right – DiD-PLGA NP, 200μm intervals, scale - 200 μm).
Figure 2
Figure 2
(A) Optical micrograph of ear skin showing microneedle penetration pattern stained using trypan blue (scale – 100μm). (B) Representative confocal z-stacks and quantification (n = 6) of (PS/SPS)20-(Poly-1/Cy3-pLUC)24-coated microneedle arrays (left – brightfield, middle – before application, right – after 24 hour application, 200μm interval, scale - 200μm). (C) Representative confocal z-stacks and quantification (n = 6) of (PS/SPS)20-(Poly-1/DiI-PLGA-NP)4 coated microneedle arrays (left – brightfield, middle – before application, right – after 5 minute application, 200μm interval, scale – 200μm). Representative confocal micrographs (1 – MHC-GFP II, 2 – Cy3-pLUC, 3 – DiI/D-PLGA NP, 4 – overlay, scale – 200μm) showing dorsal ear skin following (D) 5 minute and (E) 24 hour application of a (PS/SPS)20-(Poly-1/Cy3-pLUC)24 coated microneedle array, (F) 5 minute (PS/SPS)20-(Poly-1/DiI-PLGA-NP)4 coated microneedle application, and (G) 24 hour (PS/SPS)20-(Poly-1/Cy3-pLUC)24-(Poly-1/DiD-PLGA NP)4 coated microneedle application.
Figure 3
Figure 3
In vivo bioluminescent signal observed in C57BL/6 mice (n = 3) following treatment with a (PS/SPS)20-(Poly-1/pLUC)n – coated microneedle array to the right ear (denoted by arrow): (A) 24 bilayers for 5 minutes, (B) 1 bilayer for 24 hours, (C) 5 bilayers for 24 hours, and (D) 24 bilayers for 24 hours. The bioluminescent results following treatment are summarize in (E, F) for 7 days together with the negative control signal (denoted C) collected from the untreated ear, with (E) demonstrating the effect of application time and (F) showing the result of increasing pLUC dosage.
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