Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Nov 22;6(22):2509-13.
doi: 10.1002/smll.201001341.

Controlling differentiation of neural stem cells using extracellular matrix protein patterns

Aniruddh Solanki  1 , Shreyas Shah, Kevin A Memoli, Sung Young Park, Seunghun Hong, Ki-Bum Lee
Affiliations

Controlling differentiation of neural stem cells using extracellular matrix protein patterns

Aniruddh Solanki et al. Small. .
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
A schematic diagram of our approaches. A) The fabrication of ECM protein patterns and their application for NSC differentiation. B) The selective attachment of NSCs on the protein patterns and differentation into two different kinds of neural cells. C) The differentiation of NSCs into either neurons (red) or astrocytes (green) on the protein patterns. D) Increased neuronal differentiation on the grid patterns, as compared to the stripes and squares.
Figure 2
Figure 2
Growth and differentiation of NSCs on the laminin patterns. Phase contrast images show NSC attachment and growth on stripes (A1), squares (B1), and grids (C1) on Day 2 after seeding. Fluorescent images of cells stained for the neuronal marker TuJ1 (red) and nucleus (blue) show the extent of neuronal differentiation of NSCs on stripes (A2), squares (B2), and grids (C2) on Day 6 after seeding. Similarly, cells stained for astrocyte marker GFAP (green) and nucleus (blue) show the extent of glial differentiation on stripes (A3), squares (B3), and grids (C3) on Day 6 after seeding. Scale bars: 50 μm.
Figure 3
Figure 3
Quantitative comparison of the percentage of cells expressing the neuronal marker TuJ1 and astrocyte marker GFAP on laminin patterns of squares, stripes and grids. Six days after seeding, the differentiated cells were counted and plotted as a ratio of TuJ1-positive cells or GFAP-positive cells to the total number of cells (n = 3). Student’s unpaired t-test was used for evaluating the statistical significance for cells stained for TuJ1 on stripes and squares, compared to those on grids. (* = P < 0.01, ** = P < 0.001).
Figure 4
Figure 4
NSC alignment and differentiation on combinatorial ECM patterns. A) NSCs on grids of laminin express the neural stem cell marker, nestin (purple) on Day 2 after seeding, thus confirming that the NSCs are undifferentiated. B) NSCs stained for actin (green) show extensive spreading and cell–cell interactions on grid patterns of laminin on Day 2 after seeding, confirming that the NSCs, while still in the undifferentiated state, extensively interact with each other. C) SEM image of NSCs on Day 2 after seeding, showing the early alignment and extension of processes on grid patterns of laminin. D) NSCs previously shown to extend and grow on the grid patterns of laminin undergo neuronal differentiation and express the neuronal marker synapsin (pseudocolored yellow) on Day 6 after seeding. Scale bars: 20 μm.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Guilak F, Cohen DM, Estes BT, Gimble JM, Liedtke W, Chen CS. Cell Stem Cell. 2009;5:17. - PMC - PubMed
    2. Flaim CJ, Chien S, Bhatia SN. Nat Methods. 2005;2:119. - PubMed
    3. Flaim CJ, Teng D, Chien S, Bhatia SN. Stem Cells and Dev. 2008;17:29. - PubMed
    1. Gage FH, Ray J, Fisher LJ. Annu Rev Neurosci. 1995;18:159. - PubMed
    2. Gage FH. Science. 2000;287:1433. - PubMed
    1. Roberson ED, Mucke L. Science. 2006;314:781. - PMC - PubMed
    1. Bjorklund A, Lindvall O. Nat Neurosci. 2000;3:537. - PubMed
    2. Kim JH, Auerbach JM, Rodriguez-Gomez JA, Velasco I, Gavin D, Lumelsky N, Lee SH, Nguyen J, Sanchez-Pernaute R, Bankiewicz K, McKay R. Nature. 2002;418:50. - PubMed
    3. Lindvall O, Kokaia Z. Nature. 2006;441:1094. - PubMed
    1. Johansson CB, Momma S, Clarke DL, Risling M, Lendahl U, Frisen J. Cell. 1999;96:25. - PubMed
    2. Martino G, Pluchino S. Nat Rev Neurosci. 2006;7:395. - PubMed
    3. Barnabe-Heider F, Frisen J. Cell Stem Cell. 2008;3:16. - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources