Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Mar;13(3):341-9.
doi: 10.3109/14653249.2010.520312. Epub 2010 Sep 22.

Isolation and characterization of equine amniotic fluid-derived multipotent stem cells

Sang-Bum Park  1 , Min-Soo Seo, Jun-Gu Kang, Joon-Seok Chae, Kyung-Sun Kang
Affiliations

Isolation and characterization of equine amniotic fluid-derived multipotent stem cells

Sang-Bum Park et al. Cytotherapy. 2011 Mar.

Abstract

Background aims: Amniotic fluid (AF) is a well-known source of stem cells. However, there have been no reports regarding equine AF stem cells. We have isolated equine AF-derived multipotent stem cells (MSC) (eAF-MSC) and show that these cells exhibit self-renewal ability and multilineage differentiation.

Methods: AF was obtained from thoroughbred mares and mononuclear cells (MNC) were isolated by Ficoll-Paque density gradient. We measured the cumulative population doubling level (CPDL) and characterized the immunophenotype by flow cytometry. To investigate differentiation ability, a trilineage differentiation assay was conducted.

Results: eAF-MSC could be isolated and the proliferation level was high. eAF-MSC presented typical MSC phenotypic markers, as determined by flow cytometry. Moreover, eAF-MSC showed a trilineage differentiation capability.

Conclusions: Equine AF is a good source of MSC. Furthermore, eAF-MSC may be useful as a cell therapy application for horses.

PubMed Disclaimer

Publication types

LinkOut - more resources