Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2010 Sep 23:7:253.
doi: 10.1186/1743-422X-7-253.

Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma

Ajay Kumar Chaudhary  1 , Shruti Pandya, Ravi Mehrotra, Alok C Bharti, Mangal Singh, Mamta Singh
Affiliations
Comparative Study

Comparative study between the Hybrid Capture II test and PCR based assay for the detection of human papillomavirus DNA in oral submucous fibrosis and oral squamous cell carcinoma

Ajay Kumar Chaudhary et al. Virol J. .

Abstract

Background: Oral malignancy is a major global health problem. Besides the main risk factors of tobacco, smoking and alcohol, infection by human papillomavirus (HPV) and genetic alterations are likely to play an important role in these lesions. The purpose of this study was to compare the efficacy of HC-II assay and PCR for the detection of specific HPV type (HPV 16 E6) in OSMF and OSCC cases as well as find out the prevalence of the high risk HPV (HR-HPV) in these lesions.

Methods and materials: Four hundred and thirty patients of the potentially malignant and malignant oral lesions were taken from the Department of Otorhinolaryngology, Moti Lal Nehru Medical College, Allahabad, India from Sept 2007-March 2010. Of which 208 cases were oral submucous fibrosis (OSMF) and 222 cases were oral squamous cell carcinoma (OSCC). The HC-II assay and PCR were used for the detection of HR-HPV DNA.

Result: The overall prevalence of HR-HPV 16 E6 DNA positivity was nearly 26% by PCR and 27.4% by the HC-II assay in case of potentially malignant disorder of the oral lesions such as OSMF. However, in case of malignant oral lesions such as OSCC, 32.4% HPV 16 E6 positive by PCR and 31.4% by the HC-II assay. In case of OSMF, the two test gave concordant result for 42 positive samples and 154 negative samples, with an overall level of agreement of 85.4% (Cohen's kappa = 66.83%, 95% CI 0.553-0.783). The sensitivity and specificity of the test were 73.7% and 92.05% (p < 0.00). In case of OSCC, the two test gave concordant result for 61 positive samples and 152 negative samples, with an overall level of agreement of 88.3% (Cohen's kappa = 79.29, 95% CI 0.769-0.939) and the sensitivity and specificity of the test were 87.14% and 92.76% (p < 0.00).

Conclusion: This study concluded that slight difference was found between the positivity rate of HR-HPV infection detected by the HC-II and PCR assay in OSMF and OSCC cases and the HC II assay seemed to have better sensitivity in case of OSCC.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Figure 1 illustrated the oral sub mucous fibrosis (OSMF) and oral squamous cell carcinoma (OSCC) specimen collection and HPV DNA testing methods.
Figure 2
Figure 2
Detection of HPV infection by PCR in OSMF and OSCC cases. Gel figure A represent the ethidium bromide-staining in 2% agarose gel and showing presence of HPV infection in OSMF & OSCC cases with an amplicon of L1 consensus (450 bp) and Gel figure B represent the amplicon of HPV 16 E6 (506 bp). PC is positive control DNA, NC is negative control DNA, Lanes 1 to 9 are DNA samples from OSMF and OSCC cases, M = 100 bp molecular weight marker.
Figure 3
Figure 3
Demographic distribution of the patients in OSMF and OSCC cases.
Figure 4
Figure 4
Prevalence rate of HPV infection in OSMF ad OSCC cases by HC-II assay and PCR detection.
See this image and copyright information in PMC

References

    1. Warnakulasuriya S, Johnson NW, van der Waal I. Nomenclature and classification of potentially malignant disorders of the oral mucosa. J Oral Pathol Med. 2007;36(10):575–80. doi: 10.1111/j.1600-0714.2007.00582.x. - DOI - PubMed
    1. Shah B, Lewis MA, Bedi R. Oral sub mucous fibrosis 11 year-old Bangladeshi girl living in United Kingdom. Br Dent J. 2001;191(3):130–2. doi: 10.1038/sj.bdj.4801117a. - DOI - PubMed
    1. Chiu CJ, Chang ML, Chiang CP, Hahn LJ, Hsieh LL, Chen CJ. Interaction of collagen-related genes and susceptibility to betel quid-induced oral submucous fibrosis. Cancer Epidemiol Biomarkers Prev. 2002;11(7):646–53. - PubMed
    1. Mehrotra R, Pandya S, Chaudhary AK, Kumar M, Singh M. Prevalence of oral pre-malignant and malignant lesions at a tertiary level hospital in Allahabad, India. Asian Pac J Cancer Prev. 2008;9(2):263–5. - PubMed
    1. Chaudhary AK, Singh M, Sundaram S, Mehrotra R. Role of human papillomavirus and its detection in potentially malignant and malignant head and neck lesions: updated review. Head Neck Oncol. 2009;1:22. doi: 10.1186/1758-3284-1-22. - DOI - PMC - PubMed

Publication types