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. 2010 Nov;76(22):7646-9.
doi: 10.1128/AEM.01335-10. Epub 2010 Sep 24.

Molecular characterization of Cryptosporidium molnari reveals a distinct piscine clade

Oswaldo Palenzuela  1 , Pilar Alvarez-Pellitero, Ariadna Sitjà-Bobadilla
Affiliations

Molecular characterization of Cryptosporidium molnari reveals a distinct piscine clade

Oswaldo Palenzuela et al. Appl Environ Microbiol. 2010 Nov.

Abstract

Multilocus phylogenetic analysis of small-subunit (SSU) rRNA and actin from Cryptosporidium molnari clustered this species with the C. molnari-like genotype of an isolate from the guppy, although the two fish isolates seem to be distinct species. The analysis of available piscine genotypes provides some support for cladistic congruence of the genus Piscicryptosporidium, but additional piscine genotypes are needed.

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Figures

FIG. 1.
FIG. 1.
Photomicrographs of Cryptosporidium molnari in Sparus aurata stomach from toluidine blue-stained sections (A, B, E, F), fresh smears (A inset, C), and Kinyoun acid-fast-stained smear (D). (A) Panoramic view of a massive infection with abundant merogonial and gamogonial stages in epicellular positions and oocysts deep in the epithelium (arrows). Inset shows detail of an oocyst and a type-I meront. (B) Oocysts in the epithelium and in detached tissue debris. (C) Oocysts released to the lumen. (D) Kinyoun-stained smear containing oocysts. (E) Detail of meronts, macrogamonts, and microgamonts (arrow) in epicellular position. (F) Detail of a type-I meront (arrow) and other merogonial and gamogonial stages. Bars: 5 μm (inset A), 10 μm (B, C, E, F), 20 μm (A), 30 μm (D).
FIG. 2.
FIG. 2.
Cladogram of Cryptosporidium molnari and selected Cryptosporidium genotypes using neighbor-joining analysis of SSU rDNA sequences, rooted with the coccidian Eimeria bovis. The GenBank sequence accession number is provided for each sequence. Genetic distances were computed with the Kimura 2-parameter method with complete deletion of gaps. Percentage bootstrap support values above 50 are shown at the tree nodes. Scale bar represents genetic distance (nucleotide substitutions per site).
FIG. 3.
FIG. 3.
Cladogram of Cryptosporidium molnari and selected Cryptosporidium genotypes using neighbor-joining analysis of actin gene sequences, rooted with Plasmodium falciparum. The GenBank sequence accession number is provided for each sequence. Genetic distances were computed using the equal-input method for amino acid data, with pairwise deletion of ambiguous positions. Percentage bootstrap support values are shown at the tree nodes. Scale bar represents genetic distance (amino acid substitutions per site).
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