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. 2011 Dec;17(6):605-11.
doi: 10.1111/j.1755-5949.2010.00193.x. Epub 2010 Sep 28.

Sevoflurane postconditioning ameliorates oxygen-glucose deprivation-reperfusion injury in the rat hippocampus

Sheng Peng  1 , Pramood Kalikiri, George Mychaskiw 2nd, Dengxin Zhang, Yan Zhang, Gong-Jian Liu, Guang-Lei Wang, Zhi-yun Shen
Affiliations

Sevoflurane postconditioning ameliorates oxygen-glucose deprivation-reperfusion injury in the rat hippocampus

Sheng Peng et al. CNS Neurosci Ther. 2011 Dec.

Abstract

Introduction: Sevoflurane is well known to exert a neuroprotective effect through anesthetic preconditioning. However, its effects on postconditioning, a neuroprotective phenomenon following an insult, have not been well studied.

Aims: In this study, we examined the ability of sevoflurane to induce postconditioning in rat hippocampal slices, in vitro.

Results: 2%, 4%, and 6% sevoflurane reduced neurophysiologic and morphologic neuronal injury following oxygen-glucose deprivation (OGD) and reperfusion. The quantity of damaged neurons was significantly reduced on immunofluorescence staining; excitatory amino acids (Asp, Glu) increased and inhibitory amino acids (GABA) decreased significantly. The effect was concentration-dependent.

Conclusion: Postconditioning with sevoflurane reduces neuronal damage after OGD-reperfusion injury in the CA1 area of rat hippocampus, in vitro.

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Conflict of interest statement

Dr. Mychaskiw is a consultant who has received research support and is a member of the speakers bureau of Baxter Healthcare, Inc. The authors have no other conflicts of interest.

Figures

Figure 1
Figure 1
HE staining of hippocampal CA1 pyramidal neurons in group normal (A). hypoxia (B), 2% Sevo (C), 4% Sevo (D), 6% Sevo (E).(40 × 10).
Figure 2
Figure 2
Electron micro graph of the hippocaittpal CA1 pyramidal neurons in normal (A), hypora (B),2% Sevo (C), 4% Sevo (D), 6% Sevo (E).(×10,000).
Figure 3
Figure 3
Examples of propidium iodide fluorescence images of hippocampal slices. Images were acquired ĞOmin after the simulated ischemia. Bright areas indicate propidiurn iodide fluorescence (dead neurons). Bar = 500 fim. Group normal (A), hypoxia (B), 2% Sevo (C), 4% Sevo (D), 6% Sevo (E).
Figure 4
Figure 4
Examples öf extracellular evoked potential images, before OGD (A), at the end of OGD (B), 60 mitt after reoxygen and exposed to 2%Seı™ (C), 4%Seı™ (D), 6%Se™ (E).
Figure 5
Figure 5
Concentration of amino acid neurotransmitters in aCSF (pmol•L−1, formula image± s, n = 8).
See this image and copyright information in PMC

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