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. 2011 Jan;55(1):373-5.
doi: 10.1128/AAC.01124-10. Epub 2010 Sep 27.

Novel apramycin resistance gene apmA in bovine and porcine methicillin-resistant Staphylococcus aureus ST398 isolates

Andrea T Fessler  1 , Kristina Kadlec, Stefan Schwarz
Affiliations

Novel apramycin resistance gene apmA in bovine and porcine methicillin-resistant Staphylococcus aureus ST398 isolates

Andrea T Fessler et al. Antimicrob Agents Chemother. 2011 Jan.

Abstract

A novel apramycin resistance gene, apmA, was detected on the ca.-40-kb resistance plasmid pAFS11 from bovine methicillin-resistant Staphylococcus aureus (MRSA) of sequence type 398 (ST398). The apmA gene coded for a protein of 274 amino acids that was related only distantly to acetyltransferases involved in chloramphenicol or streptogramin A resistance. NsiI deletion of apmA resulted in a 16- to 32-fold decrease in the apramycin MICs. An apmA-specific PCR identified this gene in one additional bovine and four porcine MRSA ST398 isolates.

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Figures

FIG. 1.
FIG. 1.
Schematic presentation of the seven reading frames found on the 11,312-kb EcoRI fragment of pAFS11. The arrows indicate the extents and directions of transcription. A distance scale in kb is given below the map. The IS257 element is shown as a black box, with the white arrow indicating the transposase gene tnp. The MICs of apramycin and gentamicin conferred by the complete EcoRI fragment and the corresponding NsiI deletion clones are shown on the right-hand side. N, NsiI; E, EcoRI.
See this image and copyright information in PMC

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