Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2009 Sep 4;21(32-33):3399-403.
doi: 10.1002/adma.200900340.

Aldehyde-amine chemistry enables modulated biosealants with tissue-specific adhesion

Natalie Artzi  1 , Tarek Shazly, Aaron B Baker, Adriana Bon, Elazer R Edelman
Affiliations

Aldehyde-amine chemistry enables modulated biosealants with tissue-specific adhesion

Natalie Artzi et al. Adv Mater. .
No abstract available

PubMed Disclaimer

Figures

Figure 1
Figure 1
The adhesion of PEG:dextran-based adhesives depends on tissue type (heart, lung, liver, or duodenal rat biopsies) and constituent chemistry, evaluated at low (A, 8.8 wt. %), medium (B, wt.14.0 %) or high (C, wt. 20.0 %) concentrations of dextran aldehyde solid content. The moduli of the adhesive test elements increased with aldehyde content dependant on tissue type (D). The increase in modulus compared to low aldehyde content is presented above the bars for medium and high content variants.
Figure 2
Figure 2
Tissue reactivity in-vivo was assessed for subcutaneous implants of materials having low (8.8 wt. %), medium (14.0 wt. %) or high (20.0 wt. %) dextran aldehyde solid content. A subcutaneous pocket was created in anesthetized C57BL/6 mice and 200 μl of material was injected into the pocket using a syringe with mixing tip. After 9 days, the mice were sacrificed and the skin and subcutaneous tissue were harvested. The samples were snap frozen in liquid nitrogen and stored at −80°C until histological and zymographic analysis. Materials with increased aldehyde content cause an increased inflammatory response. (A) Histomorphometric analysis reveals increased fibrotic capsule thickness and inflammatory cell present in materials with high aldehyde content. (B) Gelatinase zymographic activity was increased with material aldehyde levels. (* p < 0.05 compared with 14.0% solid content).
Figure 3
Figure 3
(A) The relative aldehyde reactivity of heart, lung, liver and duodenal rat tissue were assessed though tissue sample conjugation of fluorescent aldehyde-coated microspheres. Aldehyde reactivity was lowest in the lung and heart, greater in the liver and highest in the duodenum (* p < 0.05 compared with liver and duodenum). (B) The interface between PEG:dextran (green) and various soft tissues, highlighted with rhodamine phalloidin (actin, red) and DAPI (cell nuclei, blue), varies with tissue. (C) Both the adhesive regime area (size normalized to interfacial length) and density (fluorescence intensity) demonstrate that tissues with high aldehyde reactivity form smaller and more continuous interfaces with PEG:dextran. * p < 0.05 compared with liver and duodenum. (D) Differential adhesive retention was seen when applied to different tissues. Adhesive is most stable when applied to the duodenum while those applied to the liver, lung and heart degrade faster and at an increasing rate. Degradation rate correlates with material affinity to the tissues (R=0.9, p<0.05)
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

See all "Cited by" articles

References

    1. Araki M, Tao H, Sato T, Nakajima N, Sugai H, Hyon SH, Nagayasu T, Nakamura T. J Thorac Cardiovasc Surg. 2007;(134):145. - PubMed
    1. Ekelund A, Nilsson OS. Int Orthop. 1991;(15):331. - PubMed
    1. Vauthier C, Dubernet C, Fattal E, Pinto-Alphandary H, Couvreur P. Adv Drug Deliv Rev. 2003;(55):519. - PubMed
    1. Vote BJ, Elder MJ. Clin Experiment Ophthalmol. 2000;(28):437. - PubMed
    1. van der Ham AC, Kort WJ, Weijma IM, van den Ingh HF, Jeekel H. Dis Colon Rectum. 1992;(35):884. - PubMed

LinkOut - more resources