Suppressing an anti-inflammatory cytokine reveals a strong age-dependent survival cost in mice

Abstract

Background: The central paradigm of ecological immunology postulates that selection acts on immunity as to minimize its cost/benefit ratio. Costs of immunity may arise because the energetic requirements of the immune response divert resources that are no longer available for other vital functions. In addition to these resource-based costs, mis-directed or over-reacting immune responses can be particularly harmful for the host. In spite of the potential importance of immunopathology, most studies dealing with the evolution of the immune response have neglected such non resource-based costs. To keep the immune response under control, hosts have evolved regulatory pathways that should be considered when studying the target of the selection pressures acting on immunity. Indeed, variation in regulation may strongly modulate the negative outcome of immune activation, with potentially important fitness consequences.

Methodology/principal findings: Here, we experimentally assessed the survival costs of reduced immune regulation by inhibiting an anti-inflammatory cytokine (IL-10) with anti-IL-10 receptor antibodies (anti-IL-10R) in mice that were either exposed to a mild inflammation or kept as control. The experiment was performed on young (3 months) and old (15 months) individuals, as to further assess the age-dependent cost of suppressing immune regulation. IL-10 inhibition induced high mortality in old mice exposed to the mild inflammatory insult, whereas no mortality was observed in young mice. However, young mice experienced a transitory lost in body mass when injected with the anti-IL-10R antibodies, showing that the treatment was to a lesser extent also costly for young individuals.

Conclusions: These results suggest a major role of immune regulation that deserves attention when investigating the evolution of immunity, and indicate that the capacity to down-regulate the inflammatory response is crucial for late survival and longevity.

Figure 1. Survival rate of 15 month old mice during the course of the experiment for the two treatments.

Empty dots: anti-IL-10R/PBS; full dots: anti-IL-10R/LPS; empty triangles: IgG/PBS; full triangles: IgG/LPS. None of the 3 month old mice died during the course of the experiment.

Figure 2. Temporal change in body mass of mice treated with anti-IL-10R antibodies or IgG.

(A) LPS; (B) PBS. The bars represent the mean ± SE.

Figure 3. Changes in body mass of mice treated with anti-IL-10R antibodies or IgG.

(A) young individuals; (B) old individuals. The bars represent the mean ± SE.

Figure 4. Effect of the anti-IL-10R treatment on the production of plasmatic serum amyloid A.

The bars represent the mean ± SE.

Figure 5. Effect of the LPS treatment on the production of plasmatic serum amyloid A.

The bars represent the mean ± SE.