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. 2010 Nov 8;49(46):8649-52.
doi: 10.1002/anie.201003142.

A nanoparticle size series for in vivo fluorescence imaging

Zoran Popović  1 , Wenhao Liu, Vikash P Chauhan, Jungmin Lee, Cliff Wong, Andrew B Greytak, Numpon Insin, Daniel G Nocera, Dai Fukumura, Rakesh K Jain, Moungi G Bawendi
Affiliations

A nanoparticle size series for in vivo fluorescence imaging

Zoran Popović et al. Angew Chem Int Ed Engl. .
No abstract available

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Figures

Figure 1
Figure 1
QD-based size series of particles and corresponding TEM images; a) water soluble PIL-coated QDs (10–20 nm), b) single QDs with PEGylated silica shell synthesized via reverse micelles (20–70 nm), and c) silica spheres with electrostatically assembled PIL-coated QDs followed by PEGylation for enhanced stability (100–150 nm). Scale bars are 20nm (a) and 100 nm (b, c) in length.
Figure 2
Figure 2
Dynamic light scattering (DLS) data for water solutions of PEGylated particles. Peak values from left to right: PIL-coated QDs (–, diameter 10.7 ± 2.4 nm), inverse-micelle QD-silica-PEG5000 (---, diameter 21.6 ± 3.1 nm 91.4% by volume), inverse-micelle QD-silica-PEG5000 (•••••, diameter 56.7 ± 10.1 nm), electrostatically assembled QD-silica-PEG5000 (–•–, diameter 122.4 ± 15.6 nm).
Figure 3
Figure 3
Intravital imaging of size-dependent nanoparticle distribution in real time. Intravenous injection into a SCID mouse bearing an Mu89 melanoma in a dorsal skinfold chamber with a mixture of nanoparticles with diameters of 12 nm (476 nm emission), 60 nm (606 nm emission), and 125 nm (540 nm emission). a) Representative multiphoton microscopy image demonstrates the distribution of nanoparticles at 30 min after the injection. b) Multiphoton microscopy images demonstrate distribution of the nanoparitcle in the same resion as (a) at 120 min post-injection (red-yellow hues). The distribution at ~30 min is shown as, dotted white overlay based on 3D vascular tracing. Images are mean intensity projections of 3D volumes; scale bar – 100 μm. c) Penetration depth analysis at 60 min post-injection, with intensity profiles averaged over all 3D vectors normal to the vessels based on 3D vascular tracing. The images and analysis indicate that the 12nm particles effectively extravasate and diffuse away ~100 μm in 60 min, while the 60 nm particles remain within 10 μm of the vessel walls and the 125 nm particles do not appreciably extravasate.
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