Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2010 Oct 1;2010(10):pdb.prot5507.
doi: 10.1101/pdb.prot5507.

Culturing and egg collection of Aedes aegypti

Anthony Clemons  1 , Akio Mori, Morgan Haugen, David W Severson, Molly Duman-Scheel
Affiliations

Culturing and egg collection of Aedes aegypti

Anthony Clemons et al. Cold Spring Harb Protoc. .

Abstract

Blood-feeding mosquitoes, including the dengue and yellow fever vector Aedes aegypti, transmit many of the world's deadliest diseases. Such diseases have resurged in developing countries and pose clear threats for epidemic outbreaks in developed countries. Recent mosquito genome projects have stimulated interest in the potential for arthropod-borne disease control by genetic manipulation of vector insects, and genes that regulate development are of particular interest. This protocol describes methods for culturing Ae. aegypti and includes a procedure for egg collection that can be used in conjunction with fixation, immunohistochemistry, and in situ protocols.

PubMed Disclaimer

Conflict of interest statement

Conflicts of interest: none declared

Figures

Fig. 1
Fig. 1. Mosquito rearing cage
The cotton sleeve on the cage (seen in A) permits human access and manipulations within the cage. The netted top of the cage (top down view shown in B) allows for blood feeding prior to egg collections.
See this image and copyright information in PMC

References

    1. Christophers SR. Aedes aegypti, The yellow fever mosquito: its life history, bionomics, and structure. Cambridge University Press; Cambridge, UK: 1960.
    1. Lobo NF, Clayton JR, Fraser MJ, Kafatos FC, Collins FH. High efficiency germ-line transformation of mosquitoes. Nat Protoc. 2006;1:1312–1317. - PubMed
    1. Simanton W, Clark S, Clemons A, Jacowski C, Farrell-VanZomeren A, Beach P, Browne WE, Duman-Scheel M. Conservation of arthropod midline netrin accumulation revealed with a cross-reactive antibody provides evidence for midline cell homology. Evol Dev. 2009;11:260–268. - PMC - PubMed

Publication types

LinkOut - more resources