A molecular network for de novo generation of the apical surface and lumen
- PMID: 20890297
- PMCID: PMC2975675
- DOI: 10.1038/ncb2106
A molecular network for de novo generation of the apical surface and lumen
Abstract
To form epithelial organs cells must polarize and generate de novo an apical domain and lumen. Epithelial polarization is regulated by polarity complexes that are hypothesized to direct downstream events, such as polarized membrane traffic, although this interconnection is not well understood. We have found that Rab11a regulates apical traffic and lumen formation through the Rab guanine nucleotide exchange factor (GEF), Rabin8, and its target, Rab8a. Rab8a and Rab11a function through the exocyst to target Par3 to the apical surface, and control apical Cdc42 activation through the Cdc42 GEF, Tuba. These components assemble at a transient apical membrane initiation site to form the lumen. This Rab11a-directed network directs Cdc42-dependent apical exocytosis during lumen formation, revealing an interaction between the machineries of vesicular transport and polarization.
Conflict of interest statement
The authors declare that they have no competing financial interests
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Comment in
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Opening ahead: early steps in lumen formation revealed.Nat Cell Biol. 2010 Nov;12(11):1026-8. doi: 10.1038/ncb1110-1026. Nat Cell Biol. 2010. PMID: 21045801
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Outside in: inversion of cell polarity controls epithelial lumen formation.Dev Cell. 2014 Oct 27;31(2):140-2. doi: 10.1016/j.devcel.2014.10.011. Dev Cell. 2014. PMID: 25373773 Free PMC article.
References
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- Stenmark H. Rab GTPases as coordinators of vesicle traffic. Nat Rev Mol Cell Biol. 2009 - PubMed
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