doi: 10.1007/978-3-7091-9113-2_56.
Biochemical characterization and purification of the neuronal sodium-dependent noradrenaline transporter
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- PMID: 2089105
- DOI: 10.1007/978-3-7091-9113-2_56
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Biochemical characterization and purification of the neuronal sodium-dependent noradrenaline transporter
J Neural Transm Suppl.
1990.
Abstract
The protein properties of the neuronal sodium-dependent noradrenaline (NA) transporter of PC12 (rat pheochromocytoma) cells and of bovine adreno-medullary cells were studied by means of binding of 3H-desipramine (3H-DMI). 3H-DMI binding was decreased by proteases, phospholipase A2, by disulfide reducing agents and by the sulfhydryl-group alkylating agent N-ethylmaleimide. The NA transporter was partially purified by anion exchange and affinity chromatography. Tritiated desmethylxylamine (3H-DMX) bound irreversibly and in a DMI-sensitive manner to two PC12 membrane proteins (32kd and 53kd) which may represent components of the NA transporter.
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