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. 2010 Jan;14(1):12-7.
doi: 10.4103/0972-124X.65429.

Osteoplant acts on stem cells derived from peripheral blood

Vincenzo Sollazzo  1 , Annalisa Palmieri, Ambra Girardi, Ilaria Zollino, Giorgio Brunelli, Giuseppe Spinelli, Francesco Carinci
Affiliations

Osteoplant acts on stem cells derived from peripheral blood

Vincenzo Sollazzo et al. J Indian Soc Periodontol. 2010 Jan.

Abstract

Objectives: The osteoplant is an equine, flexible, heterologous, deantigenic, cortical, and spongy bone tissue, totally reabsorbable, used for implantation of bone tissue, to restore skeletal, even weight-bearing structures. However, how the osteoplant alters osteoblast activity to promote bone formation is poorly understood.

Materials and methods: To study how the osteoplant induces osteoblast differentiation in mesenchymal stem cells, the expression levels of bone-related genes, and mesenchymal stem cell markers are analyzed, using real time Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Results: The osteoplant causes induction of osteoblast transcriptional factors such as osterix (RUNX2), and of bone-related genes such as osteopontin (SPP1) and osteocalcin (BGLAP). In contrast the expression of ENG (CD105) is significantly decreased in stem cells treated with osteoplant, with respect to untreated cells, indicating the differentiation effect of this biomaterial on stem cells.

Conclusion: The obtained results can be relevant to better understand the molecular mechanism of bone regeneration and as a model for comparing other materials with similar clinical effects.

Keywords: Gene expression; bone; osteoplant; stem cell.

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Conflict of interest statement

Conflict of Interest: None declared.

Figures

Figure 1
Figure 1
PB-hMSCs by indirect immunofluorescence (Rodamine). Cultured cells were positive for the mesenchymal stem cell marker CD73 (a), CD90 (b), CD105 (c) and negative for the hematopoietic markers CD34 (d). Nucleuses were stained with DAPI. Original magnification ×40
Figure 2
Figure 2
Gene expression analysis of PB-hMSCs after 7 days of treatment with osteoplant
See this image and copyright information in PMC

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