Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2010 Nov 8;12(6):877-82.
doi: 10.1021/cc100118y. Epub 2010 Oct 5.

Orthogonality of SFC versus HPLC for small molecule library separation

Harold N Weller  1 , Katalin Ebinger, William Bullock, Kurt J Edinger, Mark A Hermsmeier, Steven L Hoffman, David S Nirschl, Thomas Swann, Jiang Zhao, Jeffrey Kiplinger, Paul Lefebvre
Affiliations
Comparative Study

Orthogonality of SFC versus HPLC for small molecule library separation

Harold N Weller et al. J Comb Chem. .

Abstract

Preparative HPLC and HPLC-MS are well established as the methods of choice for purification of pharmaceutical library compounds. Recent advances in supercritical fluid chromatography (SFC) have now made SFC a viable alternative to HPLC for this application. One of the potential arguments for using SFC in place of, or in addition to, HPLC is that it may offer different selectivity and thus has the potential for improved separation success rates. In this paper, we examine relative success rates for SFC and HPLC in obtaining adequate selectivity for successful separation. Our results suggest that use of SFC in addition to HPLC may result in a slight (1-2%) improvement in success rate compared to use of HPLC alone.

PubMed Disclaimer

Publication types

MeSH terms

Substances

LinkOut - more resources