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. 2011 Jan;230(1-2):74-84.
doi: 10.1016/j.jneuroim.2010.09.008.

Expression of the α7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS

Roberta Benfante  1 , Ruth Adele Antonini, Maria De Pizzol, Cecilia Gotti, Francesco Clementi, Massimo Locati, Diego Fornasari
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Expression of the α7 nAChR subunit duplicate form (CHRFAM7A) is down-regulated in the monocytic cell line THP-1 on treatment with LPS

Roberta Benfante et al. J Neuroimmunol. 2011 Jan.

Abstract

We investigated the expression of the partially duplicated α7 nAChR subunit gene in three monocytic cell lines: THP-1, U937 and Mono-Mac-6. Qualitative PCR revealed the presence of the classic α7 gene in these lineages, but real-time PCR showed the exclusive expression of α7dup. Both mRNA and protein levels were reduced in THP-1 upon LPS challenge, and it was found that transcriptional down-regulation was mediated by a direct mechanism dependent on NF-κB as its specific inhibitor parthenolide prevented the reduction in the α7dup transcript. Such precise regulation suggests that α7dup may specifically participate in the inflammatory response of the innate immune system.

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