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. 2010 Sep 21;5(2):Doc03.
doi: 10.3205/dgkh000146.

Can physical stress be measured in urine using the parameter antioxidative potential?

Hicham Benkhai  1 , Sandra Lemanski, Harald Below, Jens Uwe Heiden, Elke Below, Jürgen Lademann, Manfred Bornewasser, Theo Balz, Christine Chudaske, Axel Kramer
Affiliations

Can physical stress be measured in urine using the parameter antioxidative potential?

Hicham Benkhai et al. GMS Krankenhhyg Interdiszip. .

Abstract
in English, German

Although regular exercise is known to promote health, it is also well known that competetive sports can lead to an increase of free radical production, and thus to a drop in antioxidative potential. Thus, the present study examined the effect of competetive sports on the antioxidative potential (AOP). Using chemoluminescence, the AOP was measured in the spontaneous urine of leisure and semi-professional athletes during a training camp. Further, the parameters creatinin and uric acid were measured. It was shown that physical stress led to a drop in the antioxidant potential of up to approximately 50%. To compensate for this decline, special antioxidant food is recommended.

Regelmäßige sportliche Betätigung ist eine gesundheitsfördernde Maßnahme. Dagegen kann Leistungssport auf Grund der hohen körperlichen Belastung zu vermehrter Freisetzung von Radikalen und damit zum Abfall des antioxidativen Potentials führen. In der vorliegenden Studie sollte daher die Auswirkung von Sport auf das Antioxidative Potential (AOP) untersucht werden.

Das AOP wurde im Spontanurin von Freizeitsportlern und Leistungssportlern jeweils während eines Trainingslagers mit Hilfe der Chemolumineszenz gemessen. Weiterhin wurden die Parameter Kreatinin und Harnsäure erfasst.

Es konnte nachgewiesen werden, dass eine stärkere körperliche Belastung zu einem Abfall des antioxidativen Potentials um bis zu etwa 50% führt. Zur Kompensation erscheint eine spezielle antioxidative Kost sinnvoll.

Keywords: antioxidant capacity; antioxidant potential; physical stress; radicals; sports.

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Figures

Table 1
Table 1. Determination of AOPtotal and AOPU with the ACW kit (Analytik Jena)
Table 2
Table 2. Descriptive statistics of the results of study I (mg/g creatinine)
Table 3
Table 3. Percent of uric acid in the parameter AOPtotal
Table 4
Table 4. Percent of different compounds in the AOP (composition by [28])
Figure 1
Figure 1. Correlation between uric acid and AOPtotal
Figure 2
Figure 2. Uric acid levels, total antioxidative potential (AOPtotal) and the uric acid-independent antioxidative potential (values standardized to creatinine excretion values) in participant 12 over time
Figure 3
Figure 3. Uric-acid-independent antioxidative potential in participant 12 over time (standardized to creatinine excretion values)
Figure 4
Figure 4. Course of the uric-acid-independent antioxidative potential (AOPU) of the study manager (standardized to creatinine excretion values)
Figure 5
Figure 5. Average AOPU values of 4 competetive athletes in study II (standardized to creatinine excretion values)
Figure 6
Figure 6. Deviating course of the participant’s (study II) AOPU
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