Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
. 2011 Feb;30(2):259-64.
doi: 10.1007/s10096-010-1080-2. Epub 2010 Oct 13.

Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus

G I Andriesse  1 , S Elberts, A Vrolijk, C Verhulst, J A J W Kluytmans
Affiliations
Comparative Study

Evaluation of a fourth-generation latex agglutination test for the identification of Staphylococcus aureus

G I Andriesse et al. Eur J Clin Microbiol Infect Dis. 2011 Feb.

Abstract

In this study, we evaluated a fourth-generation agglutination assay (Staph Plus; DiaMondiaL[DML]) for the rapid identification of Staphylococcus aureus. First, comparison with three third-generation assays (Slidex Staph Plus, bioMérieux; Staphaurex Plus, Murex Diagnostics; Pastorex Staph-Plus, Sanofi Diagnostics Pasteur) was performed on a predefined strain collection: 265 coagulase-negative staphylococci (CNS), 266 methicillin-resistant S. aureus (MRSA) and 262 methicillin-susceptible S. aureus (MSSA) strains ("strain study"). Second, patient material-derived strains (883 CNS, 847 MSSA and 135 MRSA) were tested concurrently with both the DML and Slidex assays ("daily practice study"). In the strain study, the overall sensitivity and specificity of the DML, Slidex, Staphaurex and Pastorex assays were 99.2% and 100%, 98.1% and 100%, 95.2% and 100%, and 98.2% and 98.8%, respectively. Using the respective tests, the result was indeterminate in 0.0%, 0.6%, 0.4% and 1.5% of the strains. Overall, the sensitivity of the DML and Slidex assays were comparable in both sub-studies. However, in MRSA strains, the sensitivity of the DML assay was significantly lower than the Slidex assay. The specificity of the Slidex assay was significantly higher than the DML assay. However, the percentage of indeterminate results was much higher for the Slidex than the DML assay. In conclusion, the presumptive identification of S. aureus by the DML assay proved to be equal to third-generation latex agglutination assays.

PubMed Disclaimer

References

    1. Essers L, Radebold K. Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test. J Clin Microbiol. 1980;12:641–643. - PMC - PubMed
    1. Verdier I, Durand G, Bes M, Taylor KL, Lina G, Vandenesch F, Fattom AI, Etienne J. Identification of the capsular polysaccharides in Staphylococcus aureus clinical isolates by PCR and agglutination tests. J Clin Microbiol. 2007;45:725–729. doi: 10.1128/JCM.01572-06. - DOI - PMC - PubMed
    1. Fournier JM, Bouvet A, Mathieu D, Nato F, Boutonnier A, Gerbal R, Brunengo P, Saulnier C, Sagot N, Slizewicz B. New latex reagent using monoclonal antibodies to capsular polysaccharide for reliable identification of both oxacillin-susceptible and oxacillin-resistant Staphylococcus aureus. J Clin Microbiol. 1993;31:1342–1344. - PMC - PubMed
    1. van Griethuysen A, Pouw M, van Leeuwen N, Heck M, Willemse P, Buiting A, Kluytmans J. Rapid slide latex agglutination test for detection of methicillin resistance in Staphylococcus aureus. J Clin Microbiol. 1999;37:2789–2792. - PMC - PubMed
    1. van Griethuysen A, Bes M, Etienne J, Zbinden R, Kluytmans J. International multicenter evaluation of latex agglutination tests for identification of Staphylococcus aureus. J Clin Microbiol. 2001;39:86–89. doi: 10.1128/JCM.39.1.86-89.2001. - DOI - PMC - PubMed

MeSH terms