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Comparative Study
. 2010 Dec;48(12):4598-601.
doi: 10.1128/JCM.00004-10. Epub 2010 Oct 13.

Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus

Surbhi Malhotra-Kumar  1 , Liesbet Van Heirstraeten, Andie Lee, José Cortinas Abrahantes, Christine Lammens, Evelyn Vanhommerig, Geert Molenberghs, Marc Aerts, Stephan Harbarth, Herman Goossens; MOSAR WP2 Study Team
Collaborators, Affiliations
Comparative Study

Evaluation of molecular assays for rapid detection of methicillin-resistant Staphylococcus aureus

Surbhi Malhotra-Kumar et al. J Clin Microbiol. 2010 Dec.

Abstract

The diagnostic sensitivities of the BD GeneOhm and Cepheid Xpert assays were compared using culture on log-serial dilutions of well-characterized methicillin-resistant Staphylococcus aureus (MRSA) and non-MRSA strains and on nasal and groin swabs from patients with histories of MRSA carriage. The sensitivities of GeneOhm and Xpert were high at 10(3)-CFU/ml MRSA concentrations (92.3% and 96.3%, respectively) although decreased considerably (<35%) at a 1-log-lower concentration. Unexpectedly, both assays also detected select coagulase-negative staphylococci, which requires further evaluation.

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