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. 2010 Sep-Oct;24(5):705-7.

A simple method to record parietal cells in the fundic mucosa in baboons

Carlos A Rubio  1 , Michael Owston, Abiel Orrego, Edward J Dick Jr
Affiliations

A simple method to record parietal cells in the fundic mucosa in baboons

Carlos A Rubio et al. In Vivo. 2010 Sep-Oct.

Abstract

Background: Gastric parietal cells in a baboon were recently found to be auto-fluorescent.

Aim: To study gastric sections with a fluorescent microscope in a cohort of baboons.

Material and methods: Gastric sections from 38 baboons were stained with hematoxylin-eosin (H&E) and examined in a fluorescence microscope (FLM). The thickness of the parietal cell population was assessed at x 10 magnification.

Results: H&E stained all mucosal cells: fovelar, parietal and chief cells. When the same sections were analyzed with an FLM, only parietal cells were auto-fluorescent, whereas fovelar and chief cells remained non-fluorescent. Parietal cells formed a distinct, continuous auto-fluorescent band. The ratio of the auto-fluorescent parietal cell band/total mucosa ranged between 0.20 and 0.30.

Conclusion: Gastric parietal cells became auto-fluorescent when H&E-stained sections from baboon stomachs were observed with an FLM. Eosin was the stain responsible for this optical phenomenon.

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Figures

Figure 1
Figure 1
Normal fundic mucosa of baboon. Superficial, foveolar, parietal and chief cells are readily seen (H&E stain, transmitted light, ×6).
Figure 2
Figure 2
Normal fundic mucosa of baboon. Note the band of autofluorescent parietal cells. Other cells in the fundic mucosa (superficial, foveolar and chief cells) remain non-fluorescent (H&E stain, indirect light fluorescent, ×4).
See this image and copyright information in PMC

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