Comparing BRIN-BD11 culture producing insulin using different type of microcarriers

Abstract

This research was conducted to examine the growth profile, growth kinetics, and insulin-secretory responsiveness of BRIN-BD11 cells grown in optimized medium on different types of microcarriers (MCs). Comparisons were made on modified polystyrene (Hillex(®) II) and crosslinked polystyrene Plastic Plus (PP) from Solohill Engineering. The cell line producing insulin was cultured in a 25 cm(2) T-flask as control while MCs based culture was implemented in a stirred tank bioreactor with 1 L working volume. For each culture type, the viable cell number, glucose, lactate, glutamate, and insulin concentrations were measured and compared. Maximum viable cell number was obtained at 1.47 × 10(5) cell/mL for PP microcarrier (PPMCs) culture, 1.35 × 10(5) cell/mL Hillex(®) II (HIIMCs) culture and 0.95 × 10(5) cell/mL for T-flask culture, respectively. The highest insulin concentration has been produced in PPMCs culture (5.31 mg/L) compared to HIIMCs culture (2.01 mg/L) and T-flask culture (1.99 mg/L). Therefore overall observation suggested that PPMCs was likely preferred to be used for BRIN-BD11 cell culture as compared with Hillex(®) II MCs.

Fig. 1

Growth profiles of BRIN-BD11 in T-flask and on both microcarriers

Fig. 2

Cell attachment of BRIN-BD11 cells on Hillex^® II microcarrier at first 8 h

Fig. 3

Glucose, lactate, and glutamate rate in BRIN-BD11 culture for 48 h showing decreasing concentrations of glucose and increasing concentrations for both lactate and glutamate. (a) T-flask culture (b) Hillex^® II microcarrier culture (c) Plastic Plus microcarrier culture

Fig. 4

Comparison of insulin secretion in culture media between T-flask and both microcarriers. After 15-h culture duration, Plastic Plus microcarrier starts to outperform Hillex^® II microcarrier and T-flask cultures

Fig. 5

On-line controlled parameters monitoring for Hillex^® II microcarrier experiment