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. 2010 Jul;33(3):195-206.

Performance of genotypic tropism testing in clinical practice using the enhanced sensitivity version of Trofile as reference assay: results from the OSCAR Study Group

Valentina Svicher  1 , Roberta D'Arrigo, Claudia Alteri, Massimo Andreoni, Gioacchino Angarano, Andrea Antinori, Guido Antonelli, Patrizia Bagnarelli, Fausto Baldanti, Ada Bertoli, Marco Borderi, Enzo Boeri, Isabella Bonn, Bianca Bruzzone, Anna Paola Callegaro, Roberta Cammarota, Filippo Canducci, Francesca Ceccherini-Silberstein, Massimo Clementi, Antonella D'Arminio Monforte, Andrea De Luca, Antonio Di Biagio, Simona Di Gianbenedetto, Giovanni Di Perri, Massimo Di Pietro, Lavinia Fabeni, Giovanni Fadda, Massimo Galli, William Gennari, Valeria Ghisetti, Andrea Giacometti, Andrea Gori, Francesco Leoncini, Franco Maggiolo, Renato Maserati, Francesco Mazzotta, Valeria Micheli, Genny Meini, Laura Monno, Cristina Mussini, Silvia Nozza, Stefania Paolucci, Saverio Parisi, Monica Pecorari, Daniele Pizzi, Tiziana Quirino, Maria Carla Re, Giuliano Rizzardini, Rosaria Santangelo, Alessandro Soria, Francesca Stazi, Gaetana Sterrantino, Ombretta Turriziani, Claudio Viscoli, Vincenzo Vullo, Adriano Lazzarin, Carlo Federico Perno; OSCAR Study Group
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  • PMID: 20954437
Free article

Performance of genotypic tropism testing in clinical practice using the enhanced sensitivity version of Trofile as reference assay: results from the OSCAR Study Group

Valentina Svicher et al. New Microbiol. 2010 Jul.
Free article

Abstract

Objective: The goal of the OSCAR programme is to evaluate the performances of genotypic HIV-1 tropism testing in clinical practice using the enhanced sensitivity version of Trofile (ESTA) as reference-assay.

Methods: HIV-1 coreceptor-usage was assessed using plasma samples from 406 HIV-1 infected patients by ESTA and by gp120 V3 population-sequencing followed by Geno2pheno (set at a False Positive Rate [FPR] of 10% and 5%).

Results: ESTA was successful in 365 (89.9%) samples indicating R5 in 254 (69.6%), and DM/X4 in 111 (30.4% of samples (104 [28.5%] DM and 7 [1.9%] X4). Genotypic-testing successfully assessed viral tropism for all 406 samples, including the 41 with undetermined result by ESTA. Genotypic-tropism testing at a FPR of 5% and 10% was 81.1% and 78.4% concordant with ESTA, respectively. Despite a sensitivity of 48.7% and 55.9% at a FPR of 5% and 10%, respectively, a high concordance (specificity: 95.3% for FPR of 5% and 88.2% for FPR of 10%) between genotypic-tropism testing and ESTA was reached in the detection of R5-tropic viruses.

Conclusion: Our results are in line with other European studies, and support the routine use of genotypic tropism testing in clinical-settings for monitoring of HIV-1 infected patients candidate to or failing CCR5-antagonists.

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