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. 2011:684:375-82.
doi: 10.1007/978-1-60761-925-3_29.

Rubisco activase activity assays

Csengele Barta  1 , A Elizabete Carmo-Silva, Michael E Salvucci
Affiliations

Rubisco activase activity assays

Csengele Barta et al. Methods Mol Biol. 2011.

Abstract

Ribulose-1,5-bisphosphate (RuBP) carboxylase/oxygenase (Rubisco) activase functions as a mechano-chemical motor protein using the energy from ATP hydrolysis to contort the structure of its target protein, Rubisco. This action modulates the activation state of Rubisco by removing tightly-bound inhibitory sugar-phosphates from Rubisco's catalytic sites, thereby restoring the sites to catalytic competence. This chapter reports methods developed for assaying the two activities of Rubisco activase: ATP hydrolysis and Rubisco activation.

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